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Fig. 4 lost-a-fin is alk8. (A) Genomic position of the zebrafish alk8 gene and the lost-a-fin mutation laftm110. (B) Alk8 protein; the mutations found in laftm110 and lafm100 mutant Alk8 are indicated with arrowheads. TM, transmembrane domain; GS, type I receptor-specific GS domain. (C,D) alk8 expression in lafm100 mutant and wild-type sibling embryos at 36 hours post-fertilization, detected via whole-mount in situ hybridization (C), and via RT-PCR analysis, with ef1a as control (D).
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