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Fig. 2 Reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of cyclin (A2, B, D1, and E), liver-enriched transcription factor (HNF-1α, HNF-1β, HNF-3β, HNF-4α, C/EBP-α, and C/EBP-β, and oncogene (p53, c-myc, N-ras, gankyrin, and β-catenin) transcripts in normal (N) and hyperplastic liver overexpressing either zfMcl-1a (Tm) or zfBFLP1 (Tb) in 5 days postfertilization (dpf) zebrafish larvae. A: Up-regulation of zebrafish cyclin A2, D1, and E was observed in zfMcl-1a-expressing hyperplastic liver, and up-regulation of A2, B, D1, and E was observed in zfBFLP1-expressing hyperplastic liver, whereas only cyclin E mRNA was detected in normal liver. B: Up-regulation of zebrafish HNF-1α, HNF-1β, HNF-3β, HNF-4α, and C/EBP-β was observed in zfMcl-1a- and zfBFLP1-expressing hyperplastic livers, whereas down-regulation of C/EBP-β mRNA was detected in zfMcl-1a-expressing hyperplastic liver. C: Up-regulation of zebrafish p53, c-myc, N-ras, gankyrin, and β-catenin was observed in either zfBFLP1 or zfMcl-1a-expressing hyperplastic liver, whereas up-regulation of p53 mRNA detected in zfMcl-1a-expressing hyperplastic liver was not significant; β-actin was used as an internal positive control. zfMcl-1a (A, lane 3) and zfBFLP1 (A, lane 5) mRNA showed very strong expression in 5 dpf hyperplastic liver, whereas very faint expression of zfMcl-1a mRNA (A, lane 2) and a trace expression of zfBFLP1 mRNA (A, lane 4) were found in normal liver.