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Fig. 6 Effect of ectopic Sonic hedgehog expression on MyoD expression in wildtype and mutant embryos. Wild-type control embryos (A-D), wild-type embryos injected with shh RNA at the 1- to 4-cell stage (E-H), spt control embryos (I) and spt embryos injected with shh RNA (J), and ntl control embryos (K) and ntl embryos injected with shh RNA (L) were hybridized with MyoD probe. Embryonic ages of the embryos are 8 hours (A,E), 10 hours (B,F), 11 hours (K,L), 12 hours (C,G,I,J) and 14 hours (D,H). In F,L, open arrows indicate the posterior unsegmented region in which expression differs in injected wild-type and ntl embryos. Scale bar in A indicates 200 µm; all panels at same magnification.