ZFIN is now using GRCz12tu for Genomic Data
Gene
man2a1
- ID
- ZDB-GENE-060526-31
- Name
- mannosidase, alpha, class 2A, member 1
- Symbol
- man2a1 Nomenclature History
- Previous Names
-
- si:ch211-131c14.1
- Type
- protein_coding_gene
- Location
- Chr: 5 Mapping Details/Browsers
- Genome Assembly
- GRCz12tu
- Annotation Status
- Current
- Description
- Predicted to enable alpha-mannosidase activity. Predicted to be involved in N-glycan processing. Predicted to act upstream of or within mannose metabolic process. Predicted to be located in membrane. Predicted to be active in Golgi membrane. Orthologous to human MAN2A1 (mannosidase alpha class 2A member 1).
- Genome Resources
- Note
- None
- Comparative Information
-
- All Expression Data
- 1 figure from Bergen et al., 2017
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
- No data available
Wild Type Expression Summary
- All Phenotype Data
- No data available
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| a297 | Allele with multiple variants | Unknown | Unknown | CRISPR | |
| la025193Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| sa9369 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa9630 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa10617 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa31479 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa33726 | Allele with one point mutation | Unknown | Premature Stop | ENU | |
| sa44621 | Allele with one point mutation | Unknown | Unknown | ENU |
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| Targeting Reagent | Created Alleles | Citations |
|---|---|---|
| CRISPR1-man2a1 | Varshney et al., 2015 | |
| CRISPR2-man2a1 | (3) | |
| CRISPR3-man2a1 | (3) | |
| CRISPR4-man2a1 | Thyme et al., 2019 | |
| CRISPR5-man2a1 | Thyme et al., 2019 | |
| CRISPR6-man2a1 | Thyme et al., 2019 | |
| CRISPR7-man2a1 | Thyme et al., 2019 |
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Human Disease
Domain, Family, and Site Summary
| Type | InterPro ID | Name |
|---|---|---|
| Domain | IPR000602 | Glycoside hydrolase family 38, N-terminal domain |
| Domain | IPR011682 | Glycosyl hydrolase family 38, C-terminal |
| Domain | IPR015341 | Glycoside hydrolase family 38, central domain |
| Family | IPR050843 | Glycosyl Hydrolase Family 38 |
| Homologous_superfamily | IPR011013 | Galactose mutarotase-like domain superfamily |
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Domain Details Per Protein
| Protein | Additional Resources | Length | Galactose mutarotase-like domain superfamily | Glycoside hydrolase 38, N-terminal domain superfamily | Glycoside hydrolase/deacetylase, beta/alpha-barrel | Glycoside hydrolase families 57/38, central domain superfamily | Glycoside hydrolase family 38, central domain | Glycoside hydrolase family 38, central domain superfamily | Glycoside hydrolase family 38, N-terminal domain | Glycosyl hydrolase, all-beta | Glycosyl Hydrolase Family 38 | Glycosyl hydrolase family 38, C-terminal |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:A0A0R4IVS5 | InterPro | 1150 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
man2a1-201
(1)
|
Ensembl | 4,037 nt | ||
| mRNA |
man2a1-202
(1)
|
Ensembl | 1,434 nt |
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Interactions and Pathways
No data available
Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(-3.2gnat2:man2a1-GFP) |
|
| Insinna et al., 2010 | ||
| Tg(-3.2gnat2:man2a1-RFP) |
|
| Bachmann-Gagescu et al., 2011 | ||
| Tg(actb2:man2a1-EGFP) |
|
| 1 | (4) | |
| Tg(crx:man2a1-GFP) |
|
| 1 | (5) | |
| Tg(gnat2:man2a1-RFP) |
|
| 1 | Zebrafish Nomenclature Committee |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH211-131C14 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-79C1 | ZFIN Curated Data | |
| Contained in | BAC | DKEY-264M17 | ZFIN Curated Data | |
| Contained in | Fosmid | CH1073-245F10 | ZFIN Curated Data | |
| Encodes | cDNA | MGC:171587 | ZFIN Curated Data |
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| Type | Accession # | Genome Assembly | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_001110027 (1) | 4178 nt | ||
| Genomic | GenBank:CR391969 (2) | 159249 nt | ||
| Polypeptide | UniProtKB:A0A0R4IVS5 (1) | 1150 aa |
- Clark, B.S., Miesfeld, J.B., Flinn, M.A., Collery, R.F., Link, B.A. (2021) Dynamic Polarization of Rab11a Modulates Crb2a Localization and Impacts Signaling to Regulate Retinal Neurogenesis. Frontiers in cell and developmental biology. 8:608112
- Thyme, S.B., Pieper, L.M., Li, E.H., Pandey, S., Wang, Y., Morris, N.S., Sha, C., Choi, J.W., Herrera, K.J., Soucy, E.R., Zimmerman, S., Randlett, O., Greenwood, J., McCarroll, S.A., Schier, A.F. (2019) Phenotypic Landscape of Schizophrenia-Associated Genes Defines Candidates and Their Shared Functions. Cell. 177(2):478-491.e20
- Bergen, D.J.M., Stevenson, N.L., Skinner, R.E.H., Stephens, D.J., Hammond, C.L. (2017) The Golgi matrix protein giantin is required for normal cilia function in zebrafish. Biology Open. 6(8):1180-1189
- George, A.A., Hayden, S., Stanton, G.R., Brockerhoff, S.E. (2016) Arf6 and the 5'phosphatase of Synaptojanin 1 regulate autophagy in cone photoreceptors. Inside the cell. 1:117-133
- George, A.A., Hayden, S., Stanton, G.R., Brockerhoff, S.E. (2016) Arf6 and the 5'phosphatase of synaptojanin 1 regulate autophagy in cone photoreceptors. BioEssays : news and reviews in molecular, cellular and developmental biology. 38 Suppl 1:S119-35
- Pei, W., Huang, S.C., Xu, L., Pettie, K., Ceci, M.L., Sánchez, M., Allende, M.L., Burgess, S.M. (2016) Loss of Mgat5a-mediated N-glycosylation stimulates regeneration in zebrafish.. Cell regeneration (London, England). 5:3
- Varshney, G.K., Pei, W., LaFave, M.C., Idol, J., Xu, L., Gallardo, V., Carrington, B., Bishop, K., Jones, M., Li, M., Harper, U., Huang, S.C., Prakash, A., Chen, W., Sood, R., Ledin, J., Burgess, S.M. (2015) High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9. Genome research. 25(7):1030-42
- George, A.A., Hayden, S., Holzhausen, L.C., Ma, E.Y., Suzuki, S.C., Brockerhoff, S.E. (2014) Synaptojanin 1 is required for endolysosomal trafficking of synaptic proteins in cone photoreceptor inner segments. PLoS One. 9(1):e84394
- Paulus, J.D., Link, B.A. (2014) Loss of Optineurin In Vivo Results in Elevated Cell Death and Alters Axonal Trafficking Dynamics. PLoS One. 9:e109922
- Varshney, G.K., Lu, J., Gildea, D., Huang, H., Pei, W., Yang, Z., Huang, S.C., Schoenfeld, D.S., Pho, N., Casero, D., Hirase, T., Mosbrook-Davis, D.M., Zhang, S., Jao, L.E., Zhang, B., Woods, I.G., Zimmerman, S., Schier, A.F., Wolfsberg, T., Pellegrini, M., Burgess, S.M., and Lin, S. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome research. 23(4):727-735
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