ZFIN ID: ZDB-FIG-050630-7867
Thisse et al., 2001 - Expression of the zebrafish genome during embryogenesis (NIH R01 RR15402). ZFIN Direct Data Submission
Submitted By: F├╝rthauer, Maximilian, Thisse, Bernard, Thisse, Christine (Citing this work)
Thisse in situ hybridization protocol
Anatomical Terms:
Stage Range: 50%-epiboly to Bud
Probe: cb110   Quality:

Fig. 2 At the onset of gastrulation the expression of Fgf8 disappears progressively from the ventral margin and increases in dorsal marginal cells establishing a dorsal to ventral gradient of expression. At 60% epiboly a weak expression is still observed in EVL cells that are now located vegetaly to the marginal cells. Dorsally a strong expression is observed in presumptive paraxial mesodermal cell and in the deepest cells of the epiblast in direct contact with the involuted mesoderm. At mid-gastrulation, in addition to the marginal EVL and the embryo margin a novel site of expression appears in the presumptive hindbrain and the underlying cephalic paraxial mesoderm. A strong expression is also observed in ventral neurectoderm (presumptive floor plate). Expression of Fgf8 increases in presumptive hindbrain and disappears from paraxial cephalic mesoderm at late gastrula. Near the margin expression is strong in presumptive neurectodermis as well as in dorsal marginal cells (that give rise to axial structures).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fgf8a AB/TU standard conditions 50%-epiboly to Bud axis ISH
50%-epiboly to Bud EVL ISH
50%-epiboly to Bud margin ISH
50%-epiboly to Bud mesoderm ISH
50%-epiboly to Bud neural keel ISH
50%-epiboly to Bud neural plate ISH
50%-epiboly to Bud periderm ISH
50%-epiboly to Bud presumptive brain ISH
50%-epiboly to Bud presumptive cephalic mesoderm ISH
50%-epiboly to Bud presumptive segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available