Et2(E1B:GAL4FF,myl7:GFP)

ID

ZDB-ETCONSTRCT-130724-3

Name

Et2(E1B:GAL4FF,myl7:GFP)

Previous Names

• Et(bp:Gal4FF-CR) (1)
• Et2(E1b:Gal4FF, myl7:GFP)
• Et2(E1b:GAL4FF, myl7:GFP)
• Et2(E1b:GAL4FF,myl7:GFP)

Type

engineered_plasmid

Regulatory Regions

• E1B
• myl7 (1)

Coding Sequences

• GAL4FF (1)
• GFP (1)

Contains Sequences

None

Note

This enhancer trapping construct is based on pDestTol2CR3, a multisite-Gateway-based plasmid.  It contains a minimal adenovirus E1b promoter driving Gal4FF (Gal4 DNA-binding domain fused to a duplicated portion of the VP16 transcriptional activation domain; Asakawa et al., 2008) followed by the late SV40 polyA signal sequence.  Following (3’ to) the enhancer trapping construct is a transgene marker: the cmlc2 promoter driving TagRFP followed by the SV40 late polyA signal sequence; this marks the heart with red fluorescence.  The enhancer trapping construct and the transgene marker are in a tail-to-tail orientation such that they drive transcription in opposite directions.  The entire cassette (enhancer trapping construct + transgene marker) is flanked by miniTol2 transposon ends (Balciunas et al., 2006).