EST

cb514

ID
ZDB-EST-021126-24
Name
cb514
Symbol
cb514
Previous Names
None
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
oligo-dT primed 26-somite stage
Digest
PCR amplification
Insert Size
2100
Cloning Site
EcoRI-SalI
Vector Type
Plasmid
Vector
pSPORT1
Polymerase
T3 RNA polymerase
PCR Amplification
(replaces the SP6 promoter with a T3 promoter)
T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Suppliers
Quality
( Simple to use, intense expression pattern restricted to a few structures )
Protocol
Thisse in situ hybridization protocol
Note
None
Gene Expression
Directly Submitted Expression Data
6 figures (19 images) from Thisse et al., 2001 [cb514]
Marker Relationships
Sequences
Citations