BioID2

ID

ZDB-EFG-210202-2

Name

BioID2

Symbol

BioID2

Previous Names

• Aquifex aeolicus BirA R40G (1)

Type

engineered_foreign_gene

Location

Unmapped

Note

From Kim Di, et al. (2016) "Identification of protein–protein associations (PPAs) is a fundamental approach to the study of protein function, subcellular proteomes, and biological mechanisms. The proximity-dependent biotin identification (BioID) method was developed to overcome barriers imposed by conventional screening methods for PPAs (Roux et al., 2012 blue right-pointing triangle). The BioID method is based on proximity-dependent cellular biotinylation by a promiscuous bacterial biotin ligase (Escherichia coli BirA R118G, hereafter called BioID; Choi-Rhee et al., 2004 blue right-pointing triangle; Cronan, 2005 blue right-pointing triangle) fused to a bait protein to generate a history of PPAs over time in living cells (Roux et al., 2012 blue right-pointing triangle). These biotinylated proteins can be selectively isolated by conventional biotin capture methods and identified using mass spectrometry (MS) analysis (Roux et al., 2012 blue right-pointing triangle, 2013; Kim et al., 2014 blue right-pointing triangle; Mehus et al., 2016 blue right-pointing triangle)."