CRISPR

CRISPR1-ddx24

ID

ZDB-CRISPR-250808-5

Name

CRISPR1-ddx24

Previous Names

None

Target

ddx24 (1)

Sequence

5' - GAAGAGCCGTCACTGGAGCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
syu601	ddx24

Expression

Gene expression in Wild Types + CRISPR1-ddx24

No data available

Phenotype

Phenotype resulting from CRISPR1-ddx24

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ddx24

Phenotype	Fish	Conditions	Figures
angiogenic sprout filopodium decreased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
whole organism kdr expression increased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 4 from Chen et al., 2025
whole organism ddx24 expression decreased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 4 from Chen et al., 2025
central artery decreased branchiness, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
central artery branching involved in blood vessel morphogenesis disrupted, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
head ddx24 expression decreased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 4 from Chen et al., 2025
whole organism kdrl expression increased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 4 from Chen et al., 2025
head lef1 expression decreased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 4 from Chen et al., 2025
head adgra2 expression decreased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 4 from Chen et al., 2025
angiogenic sprout filopodium increased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
central artery angiogenic sprout decreased amount, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
intersegmental vessel mislocalised, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
angiogenic sprout filopodium increased length, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
intersegmental vessel branching involved in blood vessel morphogenesis disrupted, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025
whole organism dead, abnormal	ddx24^{syu601/syu601}	standard conditions	Fig. 2 from Chen et al., 2025

Citations