CRISPR

CRISPR1-stac3

ID

ZDB-CRISPR-250609-1

Name

CRISPR1-stac3

Previous Names

None

Target

stac3 (1)

Sequence

5' - AGTTCTGTGACGTCTGCGCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
biu103	stac3

Expression

Gene expression in Wild Types + CRISPR1-stac3

No data available

Phenotype

Phenotype resulting from CRISPR1-stac3

No data available

Phenotype of all Fish created by or utilizing CRISPR1-stac3

Phenotype	Fish	Conditions	Figures
whole organism ryr1a expression increased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 3 from Donaka et al., 2024
yolk cellular senescence increased process quality, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 6 from Donaka et al., 2024
whole organism myf5 expression decreased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 3 from Donaka et al., 2024
skeletal muscle cell curled, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
whole organism filamentous actin decreased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
post-vent region decreased size, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
gut lipid increased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 5 from Donaka et al., 2024
swimming behavior decreased process quality, abnormal	stac3^{biu103/biu103} (AB)	lighting conditions	Fig. 2 from Donaka et al., 2024
whole organism stac3 expression decreased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 1 from Donaka et al., 2024
skeletal muscle cell disorganized, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
skeletal muscle cell decreased width, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
skeletal muscle cell decreased thickness, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
whole organism viability, abnormal	stac3^{biu103/biu103} (AB)	chemical treatment by environment: caffeine	Fig. 6 from Donaka et al., 2024
trunk decreased size, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
skeletal muscle cell decreased size, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
whole organism mstnb expression decreased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 3 from Donaka et al., 2024
slow muscle cell ab-f59 labeling spatial pattern, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024
whole organism myod1 expression increased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 3 from Donaka et al., 2024
hatching delayed, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 2 from Donaka et al., 2024
whole organism viability, ameliorated	stac3^{biu103/biu103} (AB)	chemical treatment by environment: caffeine	Fig. 6 from Donaka et al., 2024
blood calcium(2+) decreased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 3 from Donaka et al., 2024
whole organism dead, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 6 from Donaka et al., 2024
yolk lipid increased amount, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 5 from Donaka et al., 2024
swimming behavior decreased process quality, abnormal	stac3^{biu103/biu103} (AB)	lighting conditions	Fig. 2 from Donaka et al., 2024
whole organism filamentous actin decreased length, abnormal	stac3^{biu103/biu103} (AB)	standard conditions	Fig. 4 from Donaka et al., 2024

Citations