CRISPR

CRISPR4-hsf1

ID

ZDB-CRISPR-250501-1

Name

CRISPR4-hsf1

Previous Names

None

Target

hsf1 (1)

Sequence

5' - GACCAAACTCTGGACGCTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
mb30	hsf1
mb31	hsf1
mb32	hsf1

Expression

Gene expression in Wild Types + CRISPR4-hsf1

No data available

Phenotype

Phenotype resulting from CRISPR4-hsf1

No data available

Phenotype of all Fish created by or utilizing CRISPR4-hsf1

Phenotype	Fish	Conditions	Figures
whole organism dead, abnormal	hsf1^mb30/mb30	heat shock	Fig. 6 from Xiao et al., 2025
whole organism hspa1b expression amount, ameliorated	hsf1^mb30/mb30	heat shock	Fig. 7 from Xiao et al., 2025
whole organism hspa8b expression amount, ameliorated	hsf1^mb30/mb30	heat shock	Fig. 7 from Xiao et al., 2025
whole organism dnaja expression amount, ameliorated	hsf1^mb30/mb30	heat shock	Fig. 7 from Xiao et al., 2025
whole organism dead, abnormal	hsf1^mb31/mb31	heat shock	Fig. 6 from Xiao et al., 2025
whole organism hspa1b expression amount, ameliorated	hsf1^mb31/mb31	heat shock	Fig. 7 from Xiao et al., 2025
whole organism hspa8b expression amount, ameliorated	hsf1^mb31/mb31	heat shock	Fig. 7 from Xiao et al., 2025
whole organism dnaja expression amount, ameliorated	hsf1^mb31/mb31	heat shock	Fig. 7 from Xiao et al., 2025
whole organism hspa1b expression amount, ameliorated	hsf1^mb32/mb32	heat shock	Fig. 7 from Xiao et al., 2025
whole organism dead, abnormal	hsf1^mb32/mb32	heat shock	Fig. 6 from Xiao et al., 2025
whole organism hspa8b expression amount, ameliorated	hsf1^mb32/mb32	heat shock	Fig. 7 from Xiao et al., 2025
whole organism dnaja expression amount, ameliorated	hsf1^mb32/mb32	heat shock	Fig. 7 from Xiao et al., 2025
slow muscle cell myofibril decreased thickness, abnormal	smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30	standard conditions	Fig. 9 from Xiao et al., 2025
skeletal muscle dnaja expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30	standard conditions	Fig. 8 from Xiao et al., 2025
skeletal muscle unc45b expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30	standard conditions	Fig. 8 from Xiao et al., 2025
skeletal muscle hsc70 expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30	standard conditions	Fig. 8 from Xiao et al., 2025
slow muscle cell skeletal muscle fiber development disrupted, abnormal	smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30	standard conditions	Fig. 9 from Xiao et al., 2025
skeletal muscle hspa8b expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30	standard conditions	Fig. 8 from Xiao et al., 2025
slow muscle cell myofibril decreased thickness, abnormal	smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31	standard conditions	Fig. 9 from Xiao et al., 2025
skeletal muscle dnaja expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31	standard conditions	Fig. 8 from Xiao et al., 2025
skeletal muscle unc45b expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31	standard conditions	Fig. 8 from Xiao et al., 2025
skeletal muscle hsc70 expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31	standard conditions	Fig. 8 from Xiao et al., 2025
slow muscle cell skeletal muscle fiber development disrupted, abnormal	smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31	standard conditions	Fig. 9 from Xiao et al., 2025
skeletal muscle hspa8b expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31	standard conditions	Fig. 8 from Xiao et al., 2025
slow muscle cell myofibril decreased thickness, abnormal	smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32	standard conditions	Fig. 9 from Xiao et al., 2025
skeletal muscle hspa8b expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32	standard conditions	Fig. 8 from Xiao et al., 2025
skeletal muscle hsc70 expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32	standard conditions	Fig. 8 from Xiao et al., 2025
slow muscle cell skeletal muscle fiber development disrupted, abnormal	smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32	standard conditions	Fig. 9 from Xiao et al., 2025
skeletal muscle unc45b expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32	standard conditions	Fig. 8 from Xiao et al., 2025
skeletal muscle dnaja expression amount, ameliorated	smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32	standard conditions	Fig. 8 from Xiao et al., 2025

Citations