CRISPR

CRISPR1-fam76b

ID

ZDB-CRISPR-240926-4

Name

CRISPR1-fam76b

Previous Names

None

Target

fam76b (1)

Sequence

5' - GAGGGCCAGCAGCTTTGCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

View all 5 target locations

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf4124	fam76b

Expression

Gene expression in Wild Types + CRISPR1-fam76b

No data available

Phenotype

Phenotype resulting from CRISPR1-fam76b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-fam76b

Phenotype	Fish	Conditions	Figures
whole organism tal1 expression decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 9 from Zhu et al., 2023
thymus rag1 expression decreased distribution, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 5 from Zhu et al., 2023
thymic epithelium cell decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 5 from Zhu et al., 2023
whole organism fam76b expression decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 3, Fig. 5 from Zhu et al., 2023
nucleate erythrocyte hbbe1.1 expression decreased distribution, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 6 from Zhu et al., 2023
whole organism rag1 expression decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 5 from Zhu et al., 2023
thymus decreased size, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 5 from Zhu et al., 2023
macrophage increased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	transection: caudal fin	Fig. 7 from Zhu et al., 2023
whole organism mmp9 expression increased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 8 from Zhu et al., 2023
caudal fin neutrophil increased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	transection: caudal fin	Fig. 8, Fig. 9 from Zhu et al., 2023
whole organism hbbe1.1 expression decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 8, Fig. 9 from Zhu et al., 2023
whole organism lyz expression decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 9 from Zhu et al., 2023
nucleate erythrocyte hemoglobin decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 6 from Zhu et al., 2023
whole organism viability, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 4 from Zhu et al., 2023
macrophage increased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 7 from Zhu et al., 2023
whole organism mpx expression increased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 8 from Zhu et al., 2023
whole organism myb expression decreased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 8 from Zhu et al., 2023
neutrophil increased amount, abnormal	fam76b^{zf4124/zf4124} (TU)	standard conditions	Fig. 8 from Zhu et al., 2023
macrophage increased amount, abnormal	fam76b^{zf4124/zf4124}; ihb20Tg (TU)	transection: caudal fin	Fig. 7 from Zhu et al., 2023
whole organism lyz expression amount, ameliorated	fam76b^{zf4124/zf4124}; zf4126Tg (TU)	standard conditions	Fig. 9 from Zhu et al., 2023
whole organism mmp9 expression increased amount, abnormal	fam76b^{zf4124/zf4124}; zf4126Tg (TU)	standard conditions	Fig. 9 from Zhu et al., 2023
caudal fin neutrophil amount, ameliorated	fam76b^{zf4124/zf4124}; zf4126Tg (TU)	transection: caudal fin	Fig. 9 from Zhu et al., 2023
whole organism tal1 expression increased amount, abnormal	fam76b^{zf4124/zf4124}; zf4126Tg (TU)	standard conditions	Fig. 9 from Zhu et al., 2023
whole organism hbbe1.1 expression amount, ameliorated	fam76b^{zf4124/zf4124}; zf4126Tg (TU)	standard conditions	Fig. 9 from Zhu et al., 2023

Citations