CRISPR

CRISPR9-tbx5a

ID

ZDB-CRISPR-210507-4

Name

CRISPR9-tbx5a

Previous Names

Target

Sequence

5' - GCTGTACGATGTCTACCGTG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end, the first "G" was added.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
tpl147	tbx5a
tpl148	tbx5a

Expression

Gene expression in Wild Types + CRISPR9-tbx5a

No data available

Phenotype

Phenotype resulting from CRISPR9-tbx5a

No data available

Phenotype of all Fish created by or utilizing CRISPR9-tbx5a

Phenotype	Fish	Conditions	Figures
pectoral fin absent, abnormal	tbx5a^{tpl147/tpl147} + MO4-tbx5a	standard conditions	Figure 1 from Cunningham et al., 2020
pectoral fin malformed, abnormal	tbx5a^{tpl147/tpl147} + MO4-tbx5a	standard conditions	Figure 1 from Cunningham et al., 2020
heart edematous, abnormal	tbx5a^{tpl147/tpl147} + MO4-tbx5a	standard conditions	Figure 1 from Cunningham et al., 2020
pectoral fin malformed, abnormal	tbx5a^{tpl148/tpl148} + MO4-tbx5a	standard conditions	Figure 1 from Cunningham et al., 2020
pectoral fin absent, abnormal	tbx5a^{tpl148/tpl148} + MO4-tbx5a	standard conditions	Figure 1 from Cunningham et al., 2020
heart edematous, abnormal	tbx5a^{tpl148/tpl148} + MO4-tbx5a	standard conditions	Figure 1 from Cunningham et al., 2020

Citations