CRISPR

CRISPR1-tcf12

ID

ZDB-CRISPR-210317-3

Name

CRISPR1-tcf12

Previous Names

None

Target

Sequence

5' - GGTGTGAGATGCGGTGACGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR1-tcf12

Expressed Gene	Anatomy	Figures
tcf3a	head	Fig. 4 from Davis et al., 2020
tcf3b	head	Fig. 4 from Davis et al., 2020

Phenotype

Phenotype resulting from CRISPR1-tcf12

Phenotype	Fish	Figures
head tcf3b expression decreased amount, abnormal	zf103Tg/+ + CRISPR1-tcf12	Fig. 4 from Davis et al., 2020
head tcf3a expression decreased amount, abnormal	zf103Tg/+ + CRISPR1-tcf12	Fig. 4 from Davis et al., 2020
neuron EGFP expression decreased distribution, abnormal	zf103Tg/+ + CRISPR1-tcf12	Fig. 3 from Davis et al., 2020
neuron EGFP expression spatial pattern, abnormal	zf103Tg/+ + CRISPR1-tcf12	Fig. 3 from Davis et al., 2020

Phenotype of all Fish created by or utilizing CRISPR1-tcf12

Phenotype	Fish	Conditions	Figures
neuron EGFP expression spatial pattern, abnormal	zf103Tg/+ + CRISPR1-tcf12	standard conditions	Fig. 3 from Davis et al., 2020
neuron EGFP expression decreased distribution, abnormal	zf103Tg/+ + CRISPR1-tcf12	standard conditions	Fig. 3 from Davis et al., 2020
head tcf3b expression decreased amount, abnormal	zf103Tg/+ + CRISPR1-tcf12	standard conditions	Fig. 4 from Davis et al., 2020
head tcf3a expression decreased amount, abnormal	zf103Tg/+ + CRISPR1-tcf12	standard conditions	Fig. 4 from Davis et al., 2020

Citations