CRISPR

CRISPR3-gja1b

ID

ZDB-CRISPR-210119-2

Name

CRISPR3-gja1b

Previous Names

None

Target

gja1b (1)

Sequence

5' - CCAGGCTACAAACTGGCCAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
lh10	gja1b

Expression

Gene expression in Wild Types + CRISPR3-gja1b

No data available

Phenotype

Phenotype resulting from CRISPR3-gja1b

No data available

Phenotype of all Fish created by or utilizing CRISPR3-gja1b

Phenotype	Fish	Conditions	Figures
caudal fin vein decreased diameter, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 7 from Hyland et al., 2021
heart contraction decreased rate, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 4 from Hyland et al., 2021
whole organism Ab1-gja1 labeling increased amount, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 2 from Hyland et al., 2021
atrium Ab1-gja1 labeling increased amount, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 5 from Hyland et al., 2021
fin gja1b expression increased amount, abnormal	gja1b^lh10/lh10	physical alteration: fin	Fig. 5 from Bhattacharya et al., 2020
heart gap junction increased amount, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 5 from Hyland et al., 2021
heart elongated, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 4 from Hyland et al., 2021
heart decreased size, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 4 from Hyland et al., 2021
pericardium edematous, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 4 from Hyland et al., 2021
cardiac ventricle Ab1-gja1 labeling increased amount, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 5 from Hyland et al., 2021
caudal fin artery increased diameter, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 7 from Hyland et al., 2021
fin increased length, abnormal	gja1b^lh10/lh10	physical alteration: fin	Fig. 6 from Bhattacharya et al., 2020
fin evx1 expression decreased distribution, abnormal	gja1b^lh10/lh10	physical alteration: fin	Fig. 5 from Bhattacharya et al., 2020
heart malformed, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 4 from Hyland et al., 2021
caudal fin vasculature disorganized, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 7 from Hyland et al., 2021
whole organism vegfaa expression decreased amount, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 8 from Hyland et al., 2021
heart gap junction increased size, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 5 from Hyland et al., 2021
caudal fin vasculature blood circulation disrupted, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 7 from Hyland et al., 2021
whole organism gap junction decreased functionality, abnormal	gja1b^lh10/lh10	standard conditions	Fig. 5 from Hyland et al., 2021
intersegmental vessel increased diameter, abnormal	gja1b^lh10/lh10; y1Tg	standard conditions	Fig. 6 from Hyland et al., 2021

Citations