CRISPR

CRISPR3-e2f4

ID

ZDB-CRISPR-201005-1

Name

CRISPR3-e2f4

Previous Names

None

Target

e2f4 (1)

Sequence

5' - AGATCTCAAGTTGGAACTGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ouc2022	e2f4

Expression

Gene expression in Wild Types + CRISPR3-e2f4

No data available

Phenotype

Phenotype resulting from CRISPR3-e2f4

No data available

Phenotype of all Fish created by or utilizing CRISPR3-e2f4

Phenotype	Fish	Conditions	Figures
pronephric tubule single ciliated epithelial cell increased amount, abnormal	e2f4^{ouc2022/ouc2022}	standard conditions	Fig 4 from Xie et al., 2020
pronephric proximal convoluted tubule multi-ciliated epithelial cell absent, abnormal	e2f4^{ouc2022/ouc2022}	standard conditions	Fig 4 from Xie et al., 2020
pronephric tubule multi-ciliated epithelial cell absent, abnormal	e2f4^{ouc2022/ouc2022}	standard conditions	Fig 4 from Xie et al., 2020
pronephric tubule multi-ciliated epithelial cell decreased amount, abnormal	e2f4^{ouc2022/ouc2022}	standard conditions	Fig 4 from Xie et al., 2020
pronephric tubule single ciliated epithelial cell increased amount, exacerbated	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig 4 from Xie et al., 2020
olfactory pit multi-ciliated epithelial cell absent, abnormal	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig. S5 from Xie et al., 2020
pronephric tubule multi-ciliated epithelial cell absent, abnormal	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig 4 from Xie et al., 2020
pronephric proximal convoluted tubule multi-ciliated epithelial cell absent, abnormal	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig 4 from Xie et al., 2020
maintenance of ciliary planar beating movement pattern disrupted, abnormal	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig 4 from Xie et al., 2020
pronephric tubule increased diameter, abnormal	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig 4 from Xie et al., 2020
pronephric proximal straight tubule multi-ciliated epithelial cell absent, exacerbated	e2f4^{ouc2022/ouc2022}; e2f5^{ouc2019/ouc2019}	standard conditions	Fig 4 from Xie et al., 2020

Citations