CRISPR

CRISPR1-dact2

ID

ZDB-CRISPR-200924-5

Name

CRISPR1-dact2

Previous Names

None

Target

dact2 (1)

Sequence

5' - GGAATGGACCGGGGCAGGAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
yu31	dact2

Expression

Gene expression in Wild Types + CRISPR1-dact2

No data available

Phenotype

Phenotype resulting from CRISPR1-dact2

No data available

Phenotype of all Fish created by or utilizing CRISPR1-dact2

Phenotype	Fish	Conditions	Figures
intestine ccnd1 expression decreased amount, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
integument regenerating tissue vegfaa expression increased amount, abnormal	dact2^yu31/yu31 (AB)	abrasion: integument	Fig. 4 from Kim et al., 2020
intrapancreatic duct cell population proliferation increased occurrence, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
liver dact2 expression absent, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 1 from Kim et al., 2020
intestine mmp2 expression increased amount, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
liver fibrotic, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2, Fig. 3 from Kim et al., 2020
digestive system neoplastic, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2, Fig. 3 from Kim et al., 2020
intestinal epithelium cell population proliferation increased occurrence, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
intestine vegfaa expression increased amount, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
integument regenerating tissue mmp9 expression increased amount, abnormal	dact2^yu31/yu31 (AB)	abrasion: integument	Fig. 4 from Kim et al., 2020
pancreas fibrotic, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2, Fig. 3 from Kim et al., 2020
intrahepatic bile duct epithelial cell cell population proliferation increased occurrence, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
integument regenerating tissue snai1a expression increased amount, abnormal	dact2^yu31/yu31 (AB)	abrasion: integument	Fig. 4 from Kim et al., 2020
wound healing increased rate, abnormal	dact2^yu31/yu31 (AB)	abrasion: integument	Fig. 4 from Kim et al., 2020
intestine dact2 expression absent, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 1 from Kim et al., 2020
pancreas dact2 expression absent, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 1 from Kim et al., 2020
pancreatic duct hyperplastic, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2 from Kim et al., 2020
integument regenerating tissue mmp2 expression increased amount, abnormal	dact2^yu31/yu31 (AB)	abrasion: integument	Fig. 4 from Kim et al., 2020
intestinal epithelium hyperplastic, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2 from Kim et al., 2020
intestine zeb1b expression increased amount, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
intestine mmp9 expression increased amount, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020
digestive system cell population proliferation increased occurrence, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2 from Kim et al., 2020
hepatic duct hyperplastic, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 2 from Kim et al., 2020
intestine snai1a expression increased amount, abnormal	dact2^yu31/yu31 (AB)	standard conditions	Fig. 3 from Kim et al., 2020

Citations