CRISPR

CRISPR2-vtg3

ID

ZDB-CRISPR-200812-8

Name

CRISPR2-vtg3

Previous Names

sg32_Fw (1)

Target

vtg3 (1)

Sequence

5' - AGGAAACTTCCGAATGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 5' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
inr102	vtg3

Expression

Gene expression in Wild Types + CRISPR2-vtg3

No data available

Phenotype

Phenotype resulting from CRISPR2-vtg3

No data available

Phenotype of all Fish created by or utilizing CRISPR2-vtg3

Phenotype	Fish	Conditions	Figures
yolk edematous, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
oocyte fertilization decreased rate, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 7 from Yilmaz et al., 2019
female organism liver vtg7 expression increased amount, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 4 from Yilmaz et al., 2019
whole organism decreased life span, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 8 from Yilmaz et al., 2019
female organism unfertilized egg vtg3 expression absent, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 5, Fig. 6 from Yilmaz et al., 2019
fertilized egg hatching decreased rate of occurrence, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 7 from Yilmaz et al., 2019
whole organism dead, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 8 from Yilmaz et al., 2019
post-vent region bent, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
female organism liver vtg3 expression absent, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 4, Fig. 5 from Yilmaz et al., 2019
post-vent region curved, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
ovary vtg3 expression absent, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 6 from Yilmaz et al., 2019
female organism unfertilized egg vtg7 expression increased amount, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 5 from Yilmaz et al., 2019
pericardium edematous, abnormal	vtg3^{inr102/inr102} (TU)	standard conditions	Fig. 9 from Yilmaz et al., 2019
female organism liver vtg3 expression decreased amount, abnormal	vtg3^inr102/+ (TU)	standard conditions	Fig. 4 from Yilmaz et al., 2019
female organism liver vtg7 expression increased amount, abnormal	vtg3^inr102/+ (TU)	standard conditions	Fig. 4 from Yilmaz et al., 2019

Citations