CRISPR

CRISPR12-kmt2d

ID

ZDB-CRISPR-200515-2

Name

CRISPR12-kmt2d

Previous Names

None

Target

kmt2d (1)

Sequence

5' - GTATTGACTGTGGCATGCGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zy58	kmt2d
zy59	kmt2d
zy60	kmt2d

Expression

Gene expression in Wild Types + CRISPR12-kmt2d

Expressed Gene	Anatomy	Figures
notch1b	atrium bulbus arteriosus cardiac ventricle	Fig 6 from Serrano et al., 2019

Phenotype

Phenotype resulting from CRISPR12-kmt2d

Phenotype	Fish	Figures
atrium notch1b expression spatial pattern, abnormal	um14Tg + CRISPR12-kmt2d	Fig 6 from Serrano et al., 2019
cardiac ventricle notch1b expression increased distribution, abnormal	um14Tg + CRISPR12-kmt2d	Fig 6 from Serrano et al., 2019

Phenotype of all Fish created by or utilizing CRISPR12-kmt2d

Phenotype	Fish	Conditions	Figures
head decreased size, abnormal	kmt2d^zy58/zy58 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
pericardium edematous, abnormal	kmt2d^zy58/zy58 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
whole organism anterior-posterior axis decreased length, abnormal	kmt2d^zy58/zy58 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
ethmoid cartilage morphology, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
head decreased size, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 1 , Fig 2 from Serrano et al., 2019
cranial cartilage lacks parts or has fewer parts of type palatoquadrate cartilage, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
pharyngeal arch cartilage lacks parts or has fewer parts of type hyosymplectic cartilage, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
Meckel's cartilage absent, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
ethmoid cartilage decreased length, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
basihyal cartilage absent, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
pharyngeal arch cartilage lacks parts or has fewer parts of type ceratohyal cartilage, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
whole organism rbpja expression increased amount, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 6 from Serrano et al., 2019
ventral hyoid arch malformed, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
whole organism anterior-posterior axis decreased length, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
pericardium edematous, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
ventral hyoid arch lacks parts or has fewer parts of type ceratobranchial cartilage, abnormal	kmt2d^zy59/zy59 (AB)	standard conditions	Fig 2 from Serrano et al., 2019
head decreased size, abnormal	kmt2d^zy60/zy60 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
pericardium edematous, abnormal	kmt2d^zy60/zy60 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
whole organism anterior-posterior axis decreased length, abnormal	kmt2d^zy60/zy60 (AB)	standard conditions	Fig 1 from Serrano et al., 2019
atrium notch1b expression spatial pattern, abnormal	um14Tg + CRISPR12-kmt2d	standard conditions	Fig 6 from Serrano et al., 2019
cardiac ventricle notch1b expression increased distribution, abnormal	um14Tg + CRISPR12-kmt2d	standard conditions	Fig 6 from Serrano et al., 2019
heart rbpja expression increased amount, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 6 from Serrano et al., 2019
lateral dorsal aorta ventral region lacks parts or has fewer parts of type aortic arch, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 5 from Serrano et al., 2019
heart nucleus rbpja expression increased amount, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 6 from Serrano et al., 2019
cranial blood vessel lumen decreased diameter, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
aortic arch cell population proliferation decreased occurrence, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 7 from Serrano et al., 2019
inner optic circle increased diameter, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
ventricular endocardium cell division decreased occurrence, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
aortic arch cell population proliferation decreased occurrence, ameliorated	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
dorsal ciliary vein increased diameter, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
aortic arch 1 immature, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
pericardium edematous, abnormal	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
vascular endothelium endothelial cell proliferation decreased occurrence, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
cardiac ventricle lumen obstructed, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
head muscle ab-mf20 labeling spatial pattern, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 7 from Serrano et al., 2019
heart morphology, ameliorated	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
ventral aorta misrouted, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
ventricular endocardium hypoplastic, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
cardiac ventricle hypoplastic, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
endocardium cell population proliferation occurrence, ameliorated	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
opercular artery has extra parts of type angiogenic sprout, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 5 from Serrano et al., 2019
heart contraction decreased rate, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
anterior cerebral vein misrouted, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
aortic arch morphology, ameliorated	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 , Fig 9 from Serrano et al., 2019
endocardium cell population proliferation decreased occurrence, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 7 from Serrano et al., 2019
oral region vasculature decreased size, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
sternohyoid deformed, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 7 from Serrano et al., 2019
sternohyoid deformed, abnormal	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
aortic arch angiogenesis delayed, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 5 from Serrano et al., 2019
endocardium rbpja expression spatial pattern, ameliorated	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 8 from Serrano et al., 2019
ventricular endocardium morphology, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
hypobranchial artery incomplete structure, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
cardiac ventricle volume, ameliorated	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
pharyngeal arch 3-7 lacks parts or has fewer parts of type aortic arch, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
aortic arch morphology, abnormal	kmt2d^zy59/zy59; la116Tg	control	Fig 7 , Fig 9 from Serrano et al., 2019
central artery misrouted, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
heart morphology, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 6 from Serrano et al., 2019
nasal ciliary artery increased diameter, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 from Serrano et al., 2019
pericardium edematous, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 7 from Serrano et al., 2019
head muscle ab-mf20 labeling spatial pattern, abnormal	kmt2d^zy59/zy59; la116Tg	chemical treatment by environment: DAPT	Fig 7 from Serrano et al., 2019
aortic arch 1 has extra parts of type angiogenic sprout, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 5 from Serrano et al., 2019
endocardium rbpja expression increased distribution, abnormal	kmt2d^zy59/zy59; la116Tg	control	Fig 8 from Serrano et al., 2019
ventricular endocardium separated from ventricular myocardium, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 3 from Serrano et al., 2019
opercular artery decreased size, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 5 from Serrano et al., 2019
cranial vasculature malformed, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 4 , Fig 7 from Serrano et al., 2019
aortic arch lumen decreased diameter, abnormal	kmt2d^zy59/zy59; la116Tg	standard conditions	Fig 5 from Serrano et al., 2019

Citations