CRISPR

CRISPR1-smad6a

ID

ZDB-CRISPR-190307-1

Name

CRISPR1-smad6a

Previous Names

None

Target

smad6a (1)

Sequence

5' - GGGTACAGGCGGCCCACAC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
zf4414	smad6a

Expression

Gene expression in Wild Types + CRISPR1-smad6a

No data available

Phenotype

Phenotype resulting from CRISPR1-smad6a

No data available

Phenotype of all Fish created by or utilizing CRISPR1-smad6a

Phenotype	Fish	Conditions	Figures
ventral aorta decreased diameter, abnormal	smad6a^zf4414/+; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 2 from Vanhooydonck et al., 2026
whole organism dead, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta vascular associated smooth muscle cell morphology, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta blood vessel endothelial cell rhomboid, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta dissected, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta ruptured, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
atrioventricular valve morphology, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
bulbus arteriosus inner layer collagen trimer increased distribution, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta decreased diameter, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 2 from Vanhooydonck et al., 2026
whole organism viability, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta blood vessel endothelial cell detached from ventral aorta basement membrane, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventriculo bulbo valve hypertrophic, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
aortic arch size, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
atrioventricular valve hypertrophic, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
ventral aorta blood vessel endothelial cell increased length, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 5 from Vanhooydonck et al., 2026
ventriculo bulbo valve morphology, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
ventral aorta morphology, abnormal	smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
ventral aorta decreased diameter, abnormal	smad3a^sa2363/+; smad6a^zf4414/+; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 2 from Vanhooydonck et al., 2026
whole organism viability, ameliorated	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026
atrioventricular valve morphology, abnormal	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
ventral aorta decreased diameter, abnormal	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 2 from Vanhooydonck et al., 2026
atrioventricular valve hypertrophic, abnormal	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
ventriculo bulbo valve hypertrophic, abnormal	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
ventriculo bulbo valve morphology, abnormal	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 4 from Vanhooydonck et al., 2026
ventral aorta morphology, abnormal	smad3a^{sa2363/sa2363}; smad6a^{zf4414/zf4414}; smad6b^{zf4415/zf4415}; smad3b^{zf4413/zf4413}; y1Tg (AB)	standard conditions	Fig. 3 from Vanhooydonck et al., 2026

Citations