CRISPR

CRISPR1-cybb

ID

ZDB-CRISPR-190103-6

Name

CRISPR1-cybb

Previous Names

None

Target

cybb (1)

Sequence

5' - GGAAACTTTGCTGCAAATGA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
pu22	cybb

Expression

Gene expression in Wild Types + CRISPR1-cybb

No data available

Phenotype

Phenotype resulting from CRISPR1-cybb

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cybb

Phenotype	Fish	Conditions	Figures
retina zn-8 labeling increased distribution, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 4 from Weaver et al., 2018
optic tectum zn-8 labeling decreased distribution, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 5 from Weaver et al., 2018
retinal ganglion cell axon mislocalised, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 5 from Weaver et al., 2018
optic tectum innervation decreased occurrence, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 5 from Weaver et al., 2018
retinal ganglion cell layer increased width, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 4 from Weaver et al., 2018
anterior commissure defasciculated, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 5 from Weaver et al., 2018
spinal cord axon defasciculated, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 5 from Weaver et al., 2018
midbrain axon zn-8 labeling mislocalised, abnormal	cybb^pu22/pu22 (AB)	control	Fig. 5 from Weaver et al., 2018
optic tectum innervation decreased occurrence, abnormal	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	chemical treatment by environment: hydrogen peroxide	Fig. 3 from Weaver et al., 2018
cranial nerve II thickness, ameliorated	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	chemical treatment by environment: hydrogen peroxide	Fig. 3 from Weaver et al., 2018
optic tectum GFP expression decreased distribution, abnormal	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	chemical treatment by environment: hydrogen peroxide	Fig. 3 from Weaver et al., 2018
cranial nerve II decreased thickness, abnormal	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	control	Fig. 2, Fig. 3 from Weaver et al., 2018
optic tectum GFP expression decreased distribution, abnormal	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	control	Fig. 2, Fig. 3 from Weaver et al., 2018
optic tectum innervation decreased occurrence, abnormal	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	control	Fig. 2, Fig. 3 from Weaver et al., 2018
optic tectum GFP expression spatial pattern, ameliorated	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	chemical treatment by environment: hydrogen peroxide	Fig. 3 from Weaver et al., 2018
optic tectum innervation occurrence, ameliorated	rw021Tg + CRISPR1-cybb + CRISPR2-cybb	chemical treatment by environment: hydrogen peroxide	Fig. 3 from Weaver et al., 2018

Citations