Microtubule stability is altered in Map1b-depleted embryos. a Hindbrain sections of uninjected (a1, a2), map1b MO2-injected (b1, b2) and δmap1b-injected (c1, c2) embryos at 4–5 som immunolabeled with anti-β-tub (green, a1, b1, c1) and anti-glu-tub (red, a2, b2, c2). Nuclei are labeled in blue with DAPI. Insets show higher magnification of boxed areas. Scale bars: 10 µm. b Quantification of the average number of glu-tub - labeled bundles per nucleus in uninjected, standard MO-injected, map1b MO2-injected and δmap1b-injected embryos. (*) Indicates statistical significance (P <0.05 for uninjected vs map1b MO2 and P <0.05 for uninjected vs δmap1b-injected) using ANOVA followed by a Bonferroni post test. c Quantification of the average number of β-tub labeled bundles per nucleus in uninjected, standard MO-injected, map1b MO2-injected and δmap1b-injected embryos. (*) Indicates statistical significance (P <0.01 for standard MO vs δmap1b-injected) using ANOVA followed by a Bonferroni post test (d) Quantification of the neural plate width in control (uninjected), map1b MO2-injected embryos, nocodazole (3 µM)-treated embryos, and map1b MO1-injected embryos treated with nocodazole
|
