|
Fig. 3 The NPAS3 gene contains the largest number of HAEs. In the central panel, schematic drawing modified from the UCSC genome browser, showing the NPAS3 locus in genomic context on chromosome 14q13.1. Below, the four splicing variants of the NPAS3 gene are shown. The coloured bars indicate the location of the 14 HAEs. Blue bars indicate HARs (Pollard, Salama, King, et al. 2006; Pollard, Salama, Lambert, et al. 2006); green bars indicate HACNS (Prabhakar et al. 2006); and red bars show 29-mammals HARs (2× HAR; Lindblad-Toh et al. 2011). The overlapping HAE is shown by a green–blue bar. On top and below of the central panel, fluorescent and bright field microphotographs showing representative transgenic zebrafish of each HAE expressing EGFP at 24 hpf (H, I, J, K, L, M, N, O, P, Q, R, S, T, and U) and 48 hpf (A, B, C, D, E, F, G, V, W, X, Y, Z, AA, and BB). Small boxes (U and BB) represent 250 μm.