FIGURE

Fig. 4

ID

ZDB-FIG-220628-28

Publication

Voisard et al., 2022 - CRISPR/Cas9-Mediated Constitutive Loss of VCP (Valosin-Containing Protein) Impairs Proteostasis and Leads to Defective Striated Muscle Structure and Function In Vivo

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Fig. 4

ER stress and impaired protein homeostasis in vcp^ex3/ex3 embryos. (A) Loss of Vcp leads to an accumulation of ubiquitinated proteins in vcp^ex3/ex3 embryos compared to WT controls (N = 4). (B,C) Significantly increased Hspa5 protein levels suggest ER stress in vcp^ex3/ex3 embryos (N = 4; p = 0.0286; two-tailed t-test). Error bars indicate s.d.; * p < 0.05. (B,D) Total RPS6 protein levels are unchanged in vcp^ex3/ex3 mutant embryos (N = 4; p = 0.0286; two-tailed t-test), whereas phosphoRPS6 protein levels. Error bars indicate s.d.; ns, not significant. (B,E) are significantly increased, suggesting an activation of mTORC1 signaling (N = 4; p = 0.0286; two-tailed t-test). ß-Actin was used as a loading control. Error bars indicate s.d.; * p < 0.05. ns: not significant.

Expression Data

Antibodies:	Ab1-rps6 Ab4-ub Ab12-hspa5
Fish:	WT vcp^{ulm105/ulm105}
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	vcp^{ulm105/ulm105}
Observed In:	whole organism whole organism response to endoplasmic reticulum stress
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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