FIGURE

Fig. 4

ID
ZDB-FIG-130429-8
Publication
Liu et al., 2013 - Eaf1 and Eaf2 negatively regulate canonical Wnt/β-catenin signaling
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

The N-terminus of Eaf1 or Eaf2 acts as a dominant negative. (A) (a-f) Representative morphology of embryos injected with GFP mRNA or mRNA encoding the N-terminus of Eaf1 or Eaf2 (exons 1-3) at 26 or 50 hpf. (g) Embryos were scored morphologically at 50 hpf: white, the percentage of normal embryos; gray, embryos without forebrain; black, embryos without forebrain and eyes. (B,C) six3b expression was reduced in embryos injected with mRNA encoding the N-terminus (exons 1-3) of Eaf1 or Eaf2 (B) but was normal in embryos injected with mRNA encoding the C-terminus (exons 4-6) of Eaf1 or Eaf2 (C). (D) The N-terminus (exons 1-3) of Eaf1/2 suppresses forebrain formation by promoting enhanced Wnt/β-catenin activity. (a) Representative embryos showing normal, reduced and increased expression of six3b. (b) Embryos were scored for six3b expression after injection with mRNAs at the dosages indicated. (c) Abnormal expression of six3b in embryos injected with mRNA encoding the N-terminus (exons 1-3) of Eaf1 or Eaf2 was rescued by co-injection with β-catenin1-MO (8 ng/embryo) but not with β-catenin2-MO (8 ng/embryo). Ba-c,Ca-c,Da, dorsal views, anterior to the left. mRNA injections were at 50 pg/embryo unless stated otherwise (Db).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development