FIGURE

Fig. 3

ID

ZDB-FIG-080410-14

Publication

Kiener et al., 2008 - Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos

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Fig. 3

The tjp3/zo-3 morpholino causes the loss of Tjp3/Zo-3 protein expression. Immunofluorescence of whole mount zebrafish at 1 dpf (A-D), 2 dpf (E-F), and cryosections at 6 dpf (G-H) stained with anti-Tjp3/Zo-3. Tjp3/Zo-3 protein is lost in morpholino-injected embryos from 1 dpf up to 6 dpf. The morphant phenotype correlates to the loss of Tjp3/Zo-3 since embryos selected for the lack of an apparent phenotype still express Tjp3/Zo-3 protein (data not shown). At 1 dpf there is a loss of Tjp3/Zo-3 labeling in the olfactory placodes (A, B, arrows), otic placodes (A, B arrowheads), and skin (C-F arrowheads). At later stages, Tjp3/Zo-3 protein is absent from the apical membranes of the pronephric ducts (E-H, arrows), and intestine (G, H, arrowheads).

Expression Data

Gene:	tjp3
Fish:	AB AB + MO2-tjp3
Knockdown Reagent:	MO2-tjp3
Anatomical Terms:	integument intestine olfactory placode otic vesicle posterior kidney
Stage Range:	Prim-5 to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 316(1), Kiener, T.K., Selptsova-Friedrich, I., and Hunziker, W., Tjp3/zo-3 is critical for epidermal barrier function in zebrafish embryos, 36-49, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.