CRISPR

CRISPR1-polr2d

ID
ZDB-CRISPR-210217-1
Name
CRISPR1-polr2d
Previous Names
None
Target
Sequence
5' - GCAGCAGAACGAGAGCGCGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
agu8 polr2d
Expression
Gene expression in Wild Types + CRISPR1-polr2d
No data available
Phenotype
Phenotype resulting from CRISPR1-polr2d
No data available
Phenotype of all Fish created by or utilizing CRISPR1-polr2d
Phenotype Fish Conditions Figures
whole organism dnd1 expression increased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 4 from Maeta et al., 2020
eye decreased size, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 1 with image from Maeta et al., 2020
pericardium edematous, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 1 with image from Maeta et al., 2020
whole organism dead, abnormal polr2dagu8/agu8 (AB) standard conditions text only from Maeta et al., 2020
whole organism ddx4 expression increased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 4 from Maeta et al., 2020
whole organism glra4a expression decreased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 4 from Maeta et al., 2020
whole organism hypoplastic, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 1 with image from Maeta et al., 2020
head cell death increased occurrence, abnormal polr2dagu8/agu8 (AB) control Fig. 3 with image from Maeta et al., 2020
whole organism krt4 expression decreased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 4 from Maeta et al., 2020
trunk cell death increased occurrence, abnormal polr2dagu8/agu8 (AB) control Fig. 3 with image from Maeta et al., 2020
whole organism polr2d expression decreased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 4 from Maeta et al., 2020
whole organism rpl13a expression decreased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 4 from Maeta et al., 2020
somite decreased amount, abnormal polr2dagu8/agu8 (AB) standard conditions Fig. 2 with image from Maeta et al., 2020
Citations
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