TALEN

TALEN1-esama

ID

ZDB-TALEN-170601-1

Name

TALEN1-esama

Previous Names

None

Target

esama (1)

Target Sequence 1

5' - GTTGCCTTATGAAAA - 3'

Target Sequence 2

5' - GCAGCACCACCATCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

Forward TALEN RVD (NH NG NG NH HD HD NG NG NI NG NH NI NI NI NI) Reverse TALEN RVD (NH HD NI NH HD NI HD HD NI HD HD NI NG HD NG)

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ubs19	esama

Expression

Gene expression in Wild Types + TALEN1-esama

No data available

Phenotype

Phenotype resulting from TALEN1-esama

No data available

Phenotype of all Fish created by or utilizing TALEN1-esama

Phenotype	Fish	Conditions	Figures
central artery blood vessel lumenization decreased process quality, abnormal	esama^ubs19/ubs19; ubs16Tg	control	Fig. S4 from Hübner et al., 2018
intersegmental vessel disconnected, abnormal	esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 5 from Sauteur et al., 2017
intersegmental vessel disconnected, exacerbated	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 5 from Sauteur et al., 2017
intersegmental vessel tight junction ab1-tjp1 labeling spatial pattern, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 6 from Sauteur et al., 2017
intersegmental vessel tight junction disconnected, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 6 from Sauteur et al., 2017
intersegmental vessel EGFP expression mislocalised, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 6 from Sauteur et al., 2017
intersegmental vessel ab1-tjp1 labeling mislocalised, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 5 from Sauteur et al., 2017
intersegmental vessel basal plasma membrane podxl2 expression mislocalised, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 6 from Sauteur et al., 2017
cell-cell junction maintenance disrupted, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; y1Tg	standard conditions	Fig. 6 from Sauteur et al., 2017
apical junction assembly disrupted, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 4 from Sauteur et al., 2017
actin filament organization disrupted, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 4 from Sauteur et al., 2017
endothelial tip cell cell adhesion decreased efficacy, exacerbated	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 3 , Fig. 4 from Sauteur et al., 2017
actin cytoskeleton organization disrupted, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 4 from Sauteur et al., 2017
dorsal longitudinal anastomotic vessel blood vessel development delayed, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 3 from Sauteur et al., 2017
cell adhesion involved in sprouting angiogenesis disrupted, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 4 from Sauteur et al., 2017
endothelial tip cell aggregated, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 3 from Sauteur et al., 2017
dorsal longitudinal anastomotic vessel blood vessel development delayed, exacerbated	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 4 from Sauteur et al., 2017
endothelial tip cell actin filament bundle of filopodium decreased amount, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 3 , Fig. 4 from Sauteur et al., 2017
angiogenic sprout has extra parts of type endothelial cell filopodium, abnormal	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 3 from Sauteur et al., 2017
angiogenic sprout endothelial tip cell detached from angiogenic sprout, exacerbated	cdh5^ubs8/ubs8; esama^ubs19/ubs19; s916Tg; ubs18Tg; ubs3Tg	standard conditions	Fig. 3 from Sauteur et al., 2017

Citations