FIGURE SUMMARY
Title

An improved method for precise genome editing in zebrafish using CRISPR-Cas9 technique

Authors
Gasanov, E.V., Jędrychowska, J., Pastor, M., Wiweger, M., Methner, A., Korzh, V.P.
Source
Full text @ Mol. Biol. Rep.
Fig. 1

The schematic view of zebrafish (D. rerio) kcng4b, gdap1 and ghitm mutant generation. Stop-codons are colored red, PAM-sequences are underlined and colored blue. Bold italics shows the DNA joining nucleotides after Cas9 brakes. A wild-type translation is colored green, modified one in the mutants—purple
Fig. 2

The HRM analysis of the fish generated by precise two gRNAs CRISPR-Cas9 mutagenesis. (a, b) kcng4b variant #1; (c, d) kcng4b variant #2; (e, f) gdap1; (g, h) ghitm. (a, c, e, g) Normalized melting curves; (b, d, f, h) normalized melting peaks. Green—wild type; purple, red and magenta—heterozygote precise deletion mutants; blue—minor mutant fractions
Fig. 3

The HRM analysis of the fish generated by one gRNA CRISPR-Cas9 mutagenesis. (a, b) kcng4-gRNA-1; (c, d) kcng4b-gRNA-3; (e, f) Gdap1-gRNA-1; (g, h) Ghitm-gRNA-1. (a, c, e, g) Normalized melting curves; (b, d, f, h) normalized melting peaks. Green—wild type
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Biol. Rep.
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.