FIGURE SUMMARY
Title

Zebrafish Trak proteins 1a and 2 localize to the mitochondria

Authors
Oonk, K.A., Bienvenu, L.B., Sickler, P.S., Martin, C., Nickoloff-Bybel, E., Volk, A.M., Weiser, D.C., Walsh, S.
Source
Full text @ MicroPubl Biol
Figure 1. The zebrafish Trak proteins are paralogous to each other, and EGFP-tagged Trak1a and 2 proteins localize to the mitochondria when expressed in mammalian tissue culture cells.

A. D. rerio sequences used for alignment: trak1a (GenBank XM_001921277.3), si:dkey-28e7.3-201 (ENSDARG00000074508), and trak2-201: (ENSDARG00000102471). The star represents the position of an intron in the cloned trak1a gene to generate a truncated protein (Trak1 ΔC). B. Various fish species also have three distinct genes which predominantly group with trak1a, trak2, and si:dkey-28e7.3. C. COS7 cells were transfected with zebrafish Trak1a/pEGFP, Trak2/pEGFP, or Trak1 ΔC/pEGFP plasmids using Lipofectamine 3000 (GFP). After expression, cells were fixed with methanol and immunostained to identify mitochondria using a human TOMM40 primary antibody and TRITC secondary antibody. Nuclei were counterstained with DAPI. Overlay is presented at far right. Images were collected at 630X magnification on a Zeiss LSM 700 confocal microscope, and representative images are displayed.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ MicroPubl Biol
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