Dark et al., 2020 - Functional validation of CHMP7 as an ADHD risk gene. Translational psychiatry   10:385 Full text @ Transl Psychiatry

Fig. 1 Characterisation of <italic>chmp7</italic> expression.

a Whole-mount in situ hybridisation on zebrafish at 1, 2, and 6 dpf, using DIG-labelled RNA probes specific to zebrafish chmp7. [: head, ▼: kidney. b RT-PCR for chmp7 during early zebrafish development. actb1 was amplified as a positive control. – is the no template negative control.

Fig. 2 <italic>chmp7</italic> qRT-PCR on <italic>chmp7</italic><sup>+/+</sup>, <italic>chmp7</italic><sup>+/−</sup>, and <italic>chmp7</italic><sup>−/−</sup> embryos.

Mixed linear modelling demonstrated a significant difference between genotypes (p = 0.001). Data are from three biological replicates, and is normalised to chmp7+/+ values. Centre lines = mean, error bars = ± standard error of the mean (SEM).

Fig. 3 Activity analysis of <italic>chmp7</italic><sup><italic>+/+</italic></sup> (<italic>n</italic> = 153) and <italic>chmp7</italic><sup><italic>+/−</italic></sup> (<italic>n</italic> = 131) zebrafish 6 dpf embryos over a 24-h period.

Average time spent moving in seconds each hour per genotype is displayed on the Y-axis. Data are from three biological replicates. Error bars = ± SEM.

Fig. 4 Total brain volume is decreased in <italic>chmp7</italic> heterozygotes at 6 dpf.

a Maximum intensity projections of whole brains from Tg(HuC:eGFP);chmp7+/+ (n = 12) and Tg(HuC:eGFP);chmp7+/− (n = 12) 6 dpf fish. bchmp7+/− fish had significantly reduced total brain volumes when compared to chmp7+/+ fish. Data are from three biological replicates. Centre lines = mean, error bars = ± SEM.

Fig. 5 Activity analysis of <italic>chmp7</italic><sup>+/+</sup> and <italic>chmp7</italic><sup>+/−</sup> zebrafish 6 dpf embryos over a 24-h period, both treated with either 10 µM methylphenidate (MpH) or dH<sub>2</sub>O.

Average time spent moving in seconds each hour per genotype is displayed on the Y axis. Data are from six biological replicates. Error bars = ± SEM.

Acknowledgments:
ZFIN wishes to thank the journal Translational psychiatry for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Transl Psychiatry