FIGURE SUMMARY
Title

Functional validation of CHMP7 as an ADHD risk gene

Authors
Dark, C., Williams, C., Bellgrove, M.A., Hawi, Z., Bryson-Richardson, R.J.
Source
Full text @ Transl Psychiatry
Fig. 1

Characterisation of <italic>chmp7</italic> expression.

a Whole-mount in situ hybridisation on zebrafish at 1, 2, and 6 dpf, using DIG-labelled RNA probes specific to zebrafish chmp7. [: head, ▼: kidney. b RT-PCR for chmp7 during early zebrafish development. actb1 was amplified as a positive control. – is the no template negative control.

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Day 6
Fig. 2

<italic>chmp7</italic> qRT-PCR on <italic>chmp7</italic><sup>+/+</sup>, <italic>chmp7</italic><sup>+/−</sup>, and <italic>chmp7</italic><sup>−/−</sup> embryos.

Mixed linear modelling demonstrated a significant difference between genotypes (p = 0.001). Data are from three biological replicates, and is normalised to chmp7+/+ values. Centre lines = mean, error bars = ± standard error of the mean (SEM).

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6
Fig. 3

Activity analysis of <italic>chmp7</italic><sup><italic>+/+</italic></sup> (<italic>n</italic> = 153) and <italic>chmp7</italic><sup><italic>+/−</italic></sup> (<italic>n</italic> = 131) zebrafish 6 dpf embryos over a 24-h period.

Average time spent moving in seconds each hour per genotype is displayed on the Y-axis. Data are from three biological replicates. Error bars = ± SEM.

PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 6 to Days 7-13
Fig. 4

Total brain volume is decreased in <italic>chmp7</italic> heterozygotes at 6 dpf.

a Maximum intensity projections of whole brains from Tg(HuC:eGFP);chmp7+/+ (n = 12) and Tg(HuC:eGFP);chmp7+/− (n = 12) 6 dpf fish. bchmp7+/− fish had significantly reduced total brain volumes when compared to chmp7+/+ fish. Data are from three biological replicates. Centre lines = mean, error bars = ± SEM.

PHENOTYPE:
Fish:
Observed In:
Stage: Day 6
Fig. 5

Activity analysis of <italic>chmp7</italic><sup>+/+</sup> and <italic>chmp7</italic><sup>+/−</sup> zebrafish 6 dpf embryos over a 24-h period, both treated with either 10 µM methylphenidate (MpH) or dH<sub>2</sub>O.

Average time spent moving in seconds each hour per genotype is displayed on the Y axis. Data are from six biological replicates. Error bars = ± SEM.
Fig. S4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 30-44 to Days 45-89
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Transl Psychiatry
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