FIGURE SUMMARY
Title

BEL β-Trefoil Reduces the Migration Ability of RUNX2 Expressing Melanoma Cells in Xenotransplanted Zebrafish

Authors
Valenti, M.T., Marchetto, G., Perduca, M., Tiso, N., Mottes, M., Dalle Carbonare, L.
Source
Full text @ Molecules
Figure 1

Fluorescent BEL β-trefoil purification. (A) Elution profile of fluorescent BEL β-trefoil on the IMAC column. The blue line refers to the protein elution followed spectrophotometrically at 280 nm; the green line represents the increase in the imidazole concentration along the gradient. (B) Elution profile of fluorescent BEL β-trefoil on the G75 gel filtration column, recorded at 280 nm. (C) SDS-PAGE (12%). Lane 1: molecular mass markers (BlueRay Prestained Protein Marker, Jena Bioscience); lane 2: crude extract from bacteria; lane 3: first peak from the IMAC column, corresponding to nonspecific proteins; lane 4: second peak from the IMAC column, corresponding to the fluorescent BEL β-trefoil; lane 5: pure fluorescent BEL β-trefoil present in the main peak of the G75 chromatography.
Figure 2

BEL β-trefoil injected in the yolk of zebrafish (N:15) was able to diffuse in tissues. After 5 days of injection (dpi) BEL β-trefoil was still present in zebrafish. (A) Phase-contrast photograph of zebrafish (A). (B) Fluorescence image showing the GFP-BEL β-trefoil in zebrafish. Magnification 20X.
Figure 3

The number of cells spreading was lower in BEL β-trefoil-treated zebrafish (T: n45) compared to controls (C: n38) (p < 0.05). Representative images show untreated (A) and BEL β-trefoil-treated (B) zebrafish. At 7 dpi, initial metastases (indicated by white arrowheads) are detectable in untreated zebrafish, while most cells are still in the yolk area of treated zebrafish. *P < 0.05; Magnification 20X.
Figure 4

In untreated zebrafish (A) melanoma cells migrated in 80% and remained in situ in 20% of individuals, while in the presence of BEL β-trefoil (B) melanoma cells migrated in 33% and were still in situ in 67% of transplanted zebrafish.
Figure 5

The average number of metastases was higher in untreated zebrafish (A) compared to BEL β-trefoil-treated zebrafish (B). **p < 0.01. Magnification 200X.
Figure 6

Treatment withBEL β-trefoil reduced the expression levels of both zebrafish endogenous runx2a and runx2b genes. *p < 0.05. On the contrary, alp expression was upregulated without reaching statistical significance *P < 0.05; **P < 0.01
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Molecules
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