FIGURE SUMMARY
Title

Supramolecular Nano-Encapsulation of Anabasine Reduced Its Developmental Toxicity in Zebrafish

Authors
Gao, Y., Yang, X., Wang, Z., Zhong, Z., Hu, Y., Wang, Y.
Source
Full text @ Front Chem
Figure 1

Chemical structures of (A) anabasine (ANA), (B) cucurbit[7]uril (CB[7]), and (C) beta-cyclodextrins (β-CD).
Figure 2

1H NMR spectra of ANA (50 μM) (A) without and with (B) 0.6, (C) 1.2, and (D) 2.1 equiv. CB[7] in D2O (pD = 7) at room temperature.
Figure 3

(A) UV-vis measurements of ANA (50 μM) in the presence of increasing concentration of CB[7] (up to 250 μM) in aqueous solution (pH = 7). Inset: the fitting curve of the titration data at 260 nm; (B) Job's plot of ANA@CB[7] system (with [ANA]+[CB[7]] = 50 μM) based on a continuous variation titration in aqueous solutions (pH = 7).
Figure 4

ESI-MS result of an aqueous solution of ANA and CB[7]. The singly-charged peak at m/z = 1325.46 and the doubly-charged peak at m/z = 663.24 suggested a likely 1:1 complexation ratio of ANA@CB[7].
Figure 5

(A) Microcalorimetric measurements of ANA when titrated with CB[7] in water at room temperature. Top: Evolved thermogram during the titration. Bottom: Plot of ΔH against the molar ratio between CB[7] and ANA during the titration, with the best fit by using the “one set of binding sites” binding mode; (B) Microcalorimetric measurements of ANA when titrated with β-CD in water at room temperature. Top: Evolved thermogram of during the titration. Bottom: Plot of ΔH against the molar ratio between β-CD and ANA, with the best fit by using the “one set of binding sites” binding mode.
Figure 6

(A) The hatching percentage, and (B) survival rate of zebrafish embryos treated with ANA w and w/o CB[7] (300 μM) or β-CD (1 mM), respectively, for 2 days. Data are presented as mean ± S.E.M. (n = 25–30). *** denotes P < 0.001. # denotes that all zebrafish embryos/larva were dead.
Figure 7

Morphology of representative zebrafish larvae (72 hpf) upon treatment with ANA w and w/o CB[7]/β-CD, respectively, for 48 h. Black arrowheads point to the abnormal morphologies.
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Chem
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