FIGURE SUMMARY
Title

Expression analysis of Rab11 during zebrafish embryonic development

Authors
Zhang, H., Gao, Y., Qian, P., Dong, Z., Hao, W., Liu, D., Duan, X.
Source
Full text @ BMC Dev. Biol.

Rab11 is highly conserved during evolution. a Alignment of Rab11 protein amino acid residue sequences of Caenorhabditis elegans, Danio rerio, Oryzias latipes, Xenopus tropicalis, Gallus gallus, Mus musculus, Rattus norvegicus and Homo sapiens. The sequences accessions IDs are listed following respectively, NP_490675, NP_001251691, NP_599137, NP_477170, XP_004086096, XP_004068250, XP_004068248, NP_001016481, NP_001120303, NP_001005827, NP_001012569, NP_059078, NP_033023, NP_112414, NP_116006, NP_004654, NP_004209. These protein sequences were aligned using ClustalW2 program and edited by Jalview. The GTPase domain sequence is indicated. b Phylogenetic tree of amino acid sequences generated using the MEGA6 software

Expression of rab11 genes analyzed by RT-PCR. RT-PCR analysis of rab11a, rab11ba and rab11bb expression in embryonic zebrafish at different stages and adult tissues and organs

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: 1-cell to Day 4

Whole mount in situ and transverse section hybridization analysis of rab11a in zebrafish embryos. A 64 cell, lateral view. B 12 hpf, dorsal view, red arrowheads indicate the ubiquitous expression. C 20 hpf, lateral view, red arrowheads indicate endoderm. C’ 20 hpf, dorsal view, red arrowheads indicate brain. D 24 hpf, dorsal view, red arrowheads indicate brain. D’ 24 hpf, dorsal view, red arrowheads indicate hindbrain. E 36 hpf, lateral view, red arrowhead indicates brain. F 48 hpf, lateral view, red arrowhead indicates brain. G 72 hpf, lateral view, red arrowhead indicates brain. H 4 dpf, lateral view, red arrowhead indicates brain; green arrowheads indicate pronephric duct. H’ 4 dpf, dorsal view, arrowhead indicates brain. H” 4 dpf, lateral view, green arrowheads indicate pronephric duct. I 48 hpf, the trunk transverse section of the embryonic forebrain, arrowheads indicate cerebral cortex. I’ 48 hpf, the trunk transverse section of the embryonic midbrain, arrowheads indicate cerebral cortex. I” 48 hpf, the trunk transverse section of the embryonic hindbrain, arrowheads indicate cerebral cortex. J 48 hpf, lateral view, embryos stained with the sense rab11a probe

Whole mount in situ and transverse section hybridization analysis of rab11ba in zebrafish embryos. A 1 k cell, lateral view. B 16 hpf, lateral view. B’ 16 hpf, dorsal view, arrowheads indicate neural tube. C 20 hpf, lateral view, arrowhead indicates brain. C’ 20 hpf, dorsal view, arrowheads indicate brain. D 24 hpf, lateral view, arrowhead indicate brain. E 36 hpf, lateral view, arrowhead indicates spinal cord. E’ 36 hpf, dorsal view, arrowhead indicates brain and head structure. F 48 hpf, lateral view, arrowhead indicates spinal cord. G 72 hpf, lateral view, arrowhead indicates brain. H 4 dpf, lateral view, red arrowhead indicates brain, green arrowhead indicates pectoral fins. H’ 4 dpf, asterisks indicate neuromasts. I 48 hpf, the trunk transverse section of the embryonic forebrain, arrowheads indicate cerebral cortex. I’ 48 hpf, the trunk transverse section of the embryonic midbrain, arrowheads indicate cerebral cortex. I” 48 hpf, the trunk transverse section of the embryonic hindbrain, arrowheads indicate cerebral cortex. J 48 hpf, lateral view, embryos stained with the sense rab11ba probe

Whole mount in situ and transverse section hybridization analysis of rab11bb in zebrafish embryos. A 64 cell, lateral view. B 20 hpf, lateral view, arrowheads indicate brain and spinal cord. C 24 hpf, lateral view, arrowheads indicate brain and spinal cord. D 36 hpf, lateral view, arrowheads indicate spinal cord. D’ 36 hpf, dorsal view, red arrowheads indicate spinal cord, green arrowheads indicate pronephric duct. E 48 hpf, lateral view, arrowhead indicates brain. E’ 48 hpf, dorsal view. F 72 hpf, lateral view, arrowhead indicates ear. F’ 72 hpf, dorsal view. G 4 dpf, lateral view, arrowhead indicates pharyngeal arches. G’ 96 hpf, dorsal view, arrowhead indicates retina. H 48 hpf, the trunk transverse section of the embryonic forebrain, arrowheads indicate cerebral cortex. H’ 48 hpf, the trunk transverse section of the embryonic midbrain, arrowheads indicate cerebral cortex. H” 48 hpf, the trunk transverse section of the embryonic hindbrain, arrowheads indicate cerebral cortex. I 48 hpf, lateral view, embryos stained with the sense rab11bb probe

Acknowledgments
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