Myc-induced ALLs comprise two distinct molecular subtypes that differ in latency and leukemia stem cell number. a A schematic of the cell transplantation screen previously used to identify differences in latency and leukemia stem cell number (LSCs) between ALL clones (Blackburn et al. [9]). ** denotes p = 0.0065, by Wilcoxon rank order test. b RNA sequencing and principal component analysis of ALLs with high and low LSCs. All samples, except for 14.1.1.1 and 8.4.1.1, were assessed as technical replicates. Group 1 leukemias are demarcated by blue shading, while group 2 leukemias have been shaded in red in panels b, c, and d. c The two molecularly-defined subgroups differ in latency and LSC frequency when assessed by Wilcoxon Rank Sum Test. d Heat map and hierarchical clustering of group 1 and group 2 leukemias showing the top 100 genes positively and negatively correlated with PC1. e Gene set enrichment analysis using genes positively and negatively correlated with PC1. Genes positively correlated with PC1 are associated with T cells while those found to be negatively correlated with PC1 were B cell associated genes