FIGURE SUMMARY
Title

Therapeutic Chemical Screen Identifies Phosphatase Inhibitors to Reconstitute PKB Phosphorylation and Cardiac Contractility in ILK-Deficient Zebrafish

Authors
Pott, A., Shahid, M., Köhler, D., Pylatiuk, C., Weinmann, K., Just, S., Rottbauer, W.
Source
Full text @ Biomolecules
Fig. 2

msq mutants show cardiac edema and reduced contractile function. msq mutants can be distinguished from their wild-type siblings at 48 h post fertilization (hpf). (A–C) Starting from this developmental stage, msq show cardiac edema (B) compared to wild-type sibling (A) and significantly decreased ventricular fractional shortening compared to wild-type controls at 72 hpf (C).

PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec
Fig. 3

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 4

Calyculin A reconstitutes contractile force, PKB phosphorylation and anf expression in msq. (A) Ventricular FS of homozygous mutant msq embryos (msq−/−) in comparison to wild-type (WT) zebrafish. Homozygous msq embryos treated with 100 nmol/L calyculin A during 4–96 hpf displayed improved contractile force (25.4 ± 18.9% (p = 0.00015) compared to embryos incubated with DMSO (2.2 ± 5.3%). (n.s.: Not significant) (B) Treatment of homozygous msq−/− embryos with 100 nmol/L calyculin A displayed an increased PKB phosphorylation (pPKB) in comparison to controls. In situ hybridization revealed that calyculin A treated homozygous msq−/− embryos (E) show, in contrast to DMSO-treated wild-type zebrafish (C) and DMSO-treated msq−/− embryos (D), an almost indistinguishable anf expression.

EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 4
Fig. 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomolecules
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.