- Title
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Evolutionarily conserved Tbx5-Wnt2/2b pathway orchestrates cardiopulmonary development.
- Authors
- Steimle, J.D., Rankin, S.A., Slagle, C.E., Bekeny, J., Rydeen, A.B., Chan, S.S., Kweon, J., Yang, X.H., Ikegami, K., Nadadur, R.D., Rowton, M., Hoffmann, A.D., Lazarevic, S., Thomas, W., Boyle Anderson, E.A.T., Horb, M.E., Luna-Zurita, L., Ho, R.K., Kyba, M., Jensen, B., Zorn, A.M., Conlon, F.L., Moskowitz, I.P.
- Source
- Full text @ Proc. Natl. Acad. Sci. USA
Initiation of the swim bladder occurs independent of tbx5a/b in zebrafish. A. RNA in situ hybridization was performed for shha in 72hpf zebrafish embryos injected with controls, tbx5a, tbx5b, or tbx5a and tbx5b morpholinos (MOs). B. Zebrafish embryos were collected at 96hpf and histologically sectioned and stained with H&E comparing heartstrings (hst) controls and homozygous mutants. C. Similarly, zebrafish embryos were collected at 96hpf and histologically sectioned and stained with H&E comparing control, tbx5a, tbx5b, and tbx5a and tbx5b MOs. In all images, the black arrows indicate the forming swim bladder. EXPRESSION / LABELING:
PHENOTYPE:
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