Nasr et al., 2018 - Identification and characterization of two zebrafish Twik related potassium channels, Kcnk2a and Kcnk2b. Scientific Reports   8:15311 Full text @ Sci. Rep.

Fig. S2

In situ hybridization on wild type zebrafish larvae at 4 days post fertilization (4 dpf) using an antisense probe against kcnk2a (A) or kcnk2b (B).

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Day 4

Fig. S3

Kcnk2a and kcnk2b transfection efficiency. (A) Brightfield image of HEK-293T transfected with kcnk2a-PIRES2 GFP. (B) GFP fluorescence image of HEK-293T transfected with kcnk2a-PIRES2 GFP. (C) Merged image of A and B. (D) Brightfield image of HEK-293T transfected with kcnk2b- PIRES2 GFP. (E) GFP fluorescence image of HEK-293T transfected with kcnk2b- PIRES2 GFP. (F) Merged image of D and E. (G) Graph showing the overall transfection efficiency of HEK-293T with either kcnk2a-PIRES2 GFP or kcnk2a- PIRES2 GFP. 8x35mm dishes were plated with 3x105 HEK-293T cells and transfected with either kcnk2a-PIRES2 GFP (4 dishes) or kcnk2a-PIRES2 GFP (4 dishes). 5 non overlapping random wide field images were obtained and the mean transfection efficiency was calculated for each dish. The data in (G) is the average of the combined means. Statistics: Students t test, NS (not significant) p>0,05.

Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.