FIGURE SUMMARY
Title

In vivo selectivity and localization of reactive oxygen species (ROS) induction by osmium anticancer complexes that circumvent platinum-resistance

Authors
Coverdale, J.P.C., Bridgewater, H.E., Song, J.I., Smith, N.A., Barry, N.P.E., Bagley, I., Sadler, P.J., Romero Canelon, I.
Source
Full text @ J. Med. Chem.
Fig. 5

Reactive oxygen species (ROS) in anaesthetized whole-mount Singapore wild-type zebrafish (Danio rerio) treated with equipotent concentrations (1 or 2× LC50) of Os-sulfonamide 1 or Os-azopyridine 10 for 96 h. Fluorescence for ROS (green) is shown superimposed onto bright field images. Embryos were stained using the green reagent of the ROS/Superoxide Detection Kit (Enzo life sciences) to detect ROS. Excitation, 458 and 488 nm; green emission for ROS, 493–550 nm. Rotenone was the positive control; 50 μM, 2 min exposure.(47)
Fig. 6

Two-color fluorescent imaging of whole mount SG-WT zebrafish (Danio rerio) treated with Os(II) sulfonamide 6 for 96 h. Fluorescence for ROS (green) and 6 (red) is shown superimposed onto bright field images. Overlapping regions (yellow) are shown. Confocal images were acquired using a Zeiss LSM880 confocal microscope. Embryos were stained using the green reagent of the ROS/Superoxide Detection Kit (Enzo life sciences) for ROS detection. Excitation, 458, 488, and 561 nm; green emission for ROS, 493–550 nm; red emission for 6, 568–750 nm. See SI for full confocal data.
Fig. S1

Reactive oxygen species (ROS) in anaesthetized whole-mount SGWT zebrafish (Danio rerio) treated with equipotent concentrations (1.0 or 2.0 × LC50) of Os-sulfonamide 1 or Os-azopyridine 10 for 96 h. Fluorescence for ROS (green) is shown superimposed onto bright field images. Confocal images were acquired using a Zeiss LSM880 confocal microscope. Embryos were stained using ROS Detection Kit (Enzo life sciences). Excitation: 458, 488 nm; green emission for ROS: 493-550 nm. Rotenone was the positive control (50 μM, 2 min).

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Med. Chem.
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