FIGURE SUMMARY
Title

Endoglin integrates BMP and Wnt signalling to induce haematopoiesis through JDP2

Authors
Baik, J., Magli, A., Tahara, N., Swanson, S.A., Koyano-Nakagawa, N., Borges, L., Stewart, R., Garry, D.J., Kawakami, Y., Thomson, J.A., Perlingeiro, R.C.
Source
Full text @ Nat. Commun.

(a,b) ENDOGLIN (ENG) and control mCherry mRNAs were injected into Tg(cmlc2:EGFP) zebrafish embryos. (a) Embryos were imaged to visualize cardiac progenitors (EGFP+ cells) at 16 h.p.f. All views are dorsal. Scale bar, 100 μm. (b) Graph bars show the fold difference of EGFP+ cells, quantified by FACS, between ENG- and mCherry-injected embryos, which show reduced frequency of cardiac progenitors upon ENG induction. Bars represent s.e.m. from three independent experiments. *P<0.05 by t-test. (cf) YSs and anterior regions of E8.5 endoglin-induced mouse embryos were collected and analysed for haematopoietic and cardiac potential, respectively. (c) FACS analyses show induction of endoglin expression in the yolk sac of E8.5 iEng mice (right panel). (d) YSs from Eng-induced and control (WT) counterparts were assessed for their haematopoietic activity. Significant increased numbers of erythroid and GEMM colonies were observed upon endoglin induction. Error bars indicate s.e.m. from two litters of E8.5 Eng-induced (n=13) and WT littermate control (n=4) YSs. *P<0.05 by t-test. (e) Representative FACS profiles for the expression of Pdgfra and Flk-1 in the anterior regions of E8.5 endoglin-induced mouse embryos and control (WT) counterparts. (f) Quantification of Pdgfra+Flk-1+ cells confirms cardiac suppression upon induction of endoglin. Bars indicate s.e.’s from two litters of E8.5 Eng-induced (n=13) and WT littermate control (n=4) embryos. *P<0.05 by t-test. (g,h) iEng ES cells were differentiated as EBs. Dox was added to EB cultures from day 2, and IWR-1 from day 4 to 6. EB cultures were analysed for cardiac and haematopoietic differentiation at days 8 and 6, respectively. (g) Western blot analyses reveal rescue of cTnI expression in Eng-induced EBs that had been treated with IWR-1. (h) Gene expression analysis for embryonic and adult globins demonstrates that IWR-1 counteracts the stimulatory effect of endoglin in erythropoiesis. Transcript levels are normalized to Gapdh. Bars indicate s.e.’s from three independent experiments.

Acknowledgments
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