FIGURE SUMMARY
Title

Forebrain Control of Behaviorally Driven Social Orienting in Zebrafish

Authors
Stednitz, S.J., McDermott, E.M., Ncube, D., Tallafuss, A., Eisen, J.S., and Washbourne, P.
Source
Full text @ Curr. Biol.

Ventral Telencephalic Lesions Disrupt Social Orienting (A) Schematic of lesion technique, where a dye-coated needle is inserted into the nostril of an anesthetized zebrafish to injure the forebrain. (B) Representative image of lesion track through forebrain (dorsal view). (C) Lesion tracks localized from a subset of zebrafish, color coded to indicate severity of social deficit by location (sagittal view). (D) Representative traces of lesioned zebrafish and control stimulus fish. Dorsally lesioned zebrafish exhibit no social impairments relative to controls (p = 0.974, n = 9); however, ventral injuries result in a severe reduction in both distance from the divider and orienting behavior (∗p = 0.007, n = 7). (E) Average percent time oriented at 45°–90° for lesion experiments before (no stimulus) and after (social stimulus) social stimulus presentation. ∗p < 0.05, repeated-measures mixed model ANOVA with post-hoc simple effects tests. Horizontal bars, mean; vertical bars, ± SEM. (F) Percent time oriented over 5 min period for lesion experiments. (G) Percent time in motion for all lesion groups before and after social stimulus presentation. Horizontal bars, mean; vertical bars, ± SEM. (H) Correlation between orienting behavior and relative distance from divider in dorsally and ventrally lesioned zebrafish. Dorsally lesioned fish retain a significant correlation between orienting and distance from the divider (R2 = 0.889, ∗p < 0.001), but ventrally lesioned fish lose this relationship and more closely resemble the no-stimulus period (R2 = 0.188, p = 0.243). ∗p < 0.05, linear regression.

Chemo-genetic Ablation of Cholinergic Neurons in the Ventral Telencephalon Disrupts Social Orienting (A) Whole-brain z projections of transgenic expression in y321, y299, and dlx gal4 lines. Scale bar, 200 μm. (B) Z-projection overlay of registered brains showing expression overlap and differences in the telencephalon. Scale bar, 200 μm. (C) Representative traces and polar histograms of y321, y299, and dlx lines following nitroreductase ablation of transgene-expressing cell populations. (D) Average percent time oriented at 45°–90° for chemo-genetic ablation experiments before and after social stimulus presentation. ∗p < 0.05, repeated-measures mixed model ANOVA with post-hoc simple effects tests. Horizontal bars, mean; vertical bars, ± SEM. (E) Percent time oriented over 5 min period for chemo-genetic ablation experiments. (F) Percent time in motion for all ablation groups before (no stimulus) and after (social stimulus) social stimulus presentation. ∗p < 0.05, repeated-measures mixed model ANOVA with post-hoc simple effects tests. Horizontal bars, mean; vertical bars, ± SEM. (G) In situ hybridization images of y321:gal4;UAS:GFP neurons labeled for lhx8a transcripts. Scale bar, 100 μm. (H) In situ hybridization images of y321:gal4;UAS:GFP neurons labeled for vachtb transcripts.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.