FIGURE SUMMARY
- Title
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Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
- Authors
- Janjuha, S., Pal Singh, S., Ninov, N.
- Source
- Full text @ J. Vis. Exp.
Ex vivo live-imaging of calcium influx using GCaMP6s in zebrafish beta-cells. A primary islet from Tg(ins:nls-Renilla-mKO2); Tg(ins:GCaMP6s) zebrafish (45 dpf) was mounted in fibrinogen-thrombin mold and incubated with 5 mM (basal) glucose. Beta-cells were labeled with a red nuclear marker, while the GCaMP6s fluorescence is present in the green channel. The islet was stimulated with a glucose-ramp consisting of sequential incubation with 10 mM and 20 mM D-glucose, and depolarized via the addition of 30 mM KCl. Arrowheads mark individual beta-cells whose activity was analyzed. |
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Full text @ J. Vis. Exp.