- Title
-
Metastasis-associated miR-23a from nasopharyngeal carcinoma-derived exosomes mediates angiogenesis by repressing a novel target gene TSGA10
- Authors
- Bao, L., You, B., Shi, S., Shan, Y., Zhang, Q., Yue, H., Zhang, J., Zhang, W., Shi, Y., Liu, Y., Wang, X., Liu, D., You, Y.
- Source
- Full text @ Oncogene
MiR-23a, positively correlated with MVD in NPC, promotes angiogenesis in zebrafish. a Representative images of immunohistochemical staining for CD34 with high or low levels of miR-23a. b Pearson correlation between miR-23a expression and MVD. Linear regression. c Quantitative PCR shows that the precursor enhanced miR-23a efficiently. T test. d Images of relative embryos injected as indicated. e Nuclei of ECs in ISVs are numbered. T test. f Statistical analysis of branch point number. T test |
MiR-23a directly targets zebrafish tsga10. a The sequences of hsa-miR-23a and dre-miR-23a. Seed sequences are shown in a dashed line box. b Schematic of the predicted miR-23a binding sequence in tsga10-3′-UTR. c Overexpression of miR-23a (miR-23a-pre) reduced tsga10-3′-UTR luciferase activity in HUVECs. One-way ANOVA. d miR-23a-precursor injection reduced the mCherry levels in mCherry-tsga10-3′-UTR sensor, whereas no change in EGFP was observed. In the mutant sensor, mcherry levels were not reduced |