Fu et al., 2017 - Competitive binding between Seryl-tRNA synthetase/YY1 complex and NFKB1 at the distal segment results in differential regulation of human vegfa promoter activity during angiogenesis. Nucleic acids research   45:2423-2437 Full text @ Nucleic Acids Res.

Fig. 8

The effects of SerRS, YY1 and NFKB1 on vascular development in zebrafish embryos. The percentages of Tg(fli1:EGFP)y1 embryos exhibiting ISV phenotypes at 72 hpf were counted. Different MOs were injected to specifically knock down the expressions of zebrafish SerRS, yy1a and nfkb, including un-injected control (n = 371), zSerRS-control-MO (4 ng; n = 99), zSerRS-MO (4 ng; n = 282), zyy1a-control-MO (3 ng; n = 111), zyy1a-MO (3 ng; n = 171), znfkb-control-MO (4 ng; n = 93), znfkb-MO (4 ng; n = 235), znfkb-MO (4 ng) plus zSerRS-MO (4 ng) (n = 182), and znfkb-MO (4 ng) plus zyy1a-MO (3 ng) (n = 192). The ISV angiogenesis of Tg(fli1:EGFP)y1embryos was observed under fluorescence microscope at 72 hpf. Three different levels of ISV angiogenesis in embryos were categorized: (A) wild-type-like (wt-like), (B) hypo-phenotype (hypo-ISV, white arrowheads) and (C) hyper-phenotype (hyper-ISV, white arrows). (D) The occurrence of different ISV phenotypes in each group, as indicated, was calculated in percentage of the total number (n) of examined embryos. DLAV: dorsal longitudinal anastomotic vessels; PAV: parachordal vessels; DA: dorsal aorta; ISV: intersegmental vessels. Scale bar: 100 μm.

Fig. S10

SerRS/YY1 complex affects angiogenesis in zebrafish embryos through the VEGFA pathway. ISV angiogenesis of zebrafish embryos from transgenic line Tg(fli1:EGFP)y1 was observed under fluorescence microscopy at 48-72 hpf after knockdown of YY1, treatment of VEGF inhibitor, and knockdown of YY1 plus treatment of VEGF inhibitor. Blood vessel development in un-injected control embryos at (A) 48 hpf and (E) 72 hpf, zyy1a-MO(4 ng)-injected embryos at (B) 48 hpf and (F) 72 hpf, VEGF inhibitor SU5416 (2.5 μM)-treated embryos at (C) 48 hpf and (G) 72 hpf, and zyy1a-MO-injected embryos combined with VEGF inhibitor at (D) 48 hpf and (H) 72 hpf was examined. Three degrees of ISV angiogenesis in embryos were categorized: wild-type-like (wt-like), hypo-phenotype (white arrowheads), and hyper-phenotype (white arrows). (I) The occurrence percentages of three degrees of ISV phenotypes among 60 examined embryos in each group, as indicated, were calculated. DLAV: dorsal longitudinal anastomotic vessels; PAV: parachordal vessels; DA: dorsal aorta; ISV: intersegmental vessels. Scale bar: 100µm.

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.