- Title
-
ZeGlobalTox: An Innovative Approach to Address Organ Drug Toxicity Using Zebrafish
- Authors
- Cornet, C., Calzolari, S., Miñana-Prieto, R., Dyballa, S., van Doornmalen, E., Rutjes, H., Savy, T., D'Amico, D., Terriente, J.
- Source
- Full text @ Int. J. Mol. Sci.
Complete ZeGlobalTox experimental setup. (A) Acute Toxicity experimental pipeline; (B) ZeGlobalTox experimental pipeline. Drugs are added from 96 hpf. Cardiotoxicity is evaluated at 100 hpf, neurotoxicity at 120 hpf, and hepatotoxicity at 132 hpf. Abbreviations: NOEC (no observed effect concentration). |
Hepatotoxicity results (A) Scheme of the experimental procedure (B). Bar graphs showing average liver area in mm. (C) Bar graphs showing the percentage of larvae presenting steatosis or yolk lipid accumulation after oil red O stain (D–F) Representative oil red O whole mount staining images of (D) DMSO, (E) EtOH and (F) APAP; black arrows point at non-affected liver (D), liver with steatosis (E), and yolk lipid retention (F), respectively. Asterisks indicate statistical significance after One-way ANOVA (liver area) or Fisher’s exact test (steatosis and yolk lipid retention): * p < 0.05; ** p < 0.01; *** p < 0.001. Black bar: negative control (B). Red bar: positive control (B). n = 20 but for DMSO n = 45. |
ZeCardio β software user pipeline. (A) Video acquisition of larvae incubated with the candidate drug; (B) Video import into the software; (C) User drawn line along the heart axis; (D–H) GUI (Graphical User Interface) display of (D) Chamber kymographs; (E) atrial and ventricular BPM (Beats Per Minute) values; (F) Distribution plot over time of atrial and ventricular BPM; (G) QTc interval and EJF (Ejection Fraction) values and (H) Cardiac arrest events; (I) Output values are presented in .csv format. Kymographs and measurements are displayed in green or blue for ventricle or atrium respectively. |
Lipid droplets on a zebrafish liver stained with Oil Red O. Steatosis is considered when three or more droplets are seen within the liver area. |