cars2 gene knockdown with Morpholinos in Danio rerio affected the migration of GnRH3 neurons.

Whole mount in situ hybridization of the control (b (b'),d,f,h) and cars2 morphants (a (a'),c,e,g) embryos with gnrh3 (a (a'),b (b')), gnrh2 (c,d), kal1a (e,f), and kal1b (g,h) probes at 56 hpf. (a,b) front views, dorsal up; (a',b',c–h) dorsal view, anterior up. Results shown were representative figures from three independent experiments and the frequency of embryos with the indicated expression patterns were shown in the bottom left corner of each panel. Red arrows indicated the affected GnRH3 neurons by knockdown cars2. Scale bar: 0.1 mm.

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