FIGURE SUMMARY
Title

Neurotoxicity of a Biopesticide Analog on Zebrafish Larvae at Nanomolar Concentrations

Authors
Nasri, A., Valverde, A.J., Roche, D.B., Desrumaux, C., Clair, P., Beyrem, H., Chaloin, L., Ghysen, A., Perrier, V.
Source
Full text @ Int. J. Mol. Sci.

Effect of cyprodinyl and A6 on the central nervous system of nbt-dsred larvae. 1-dpf embryos were exposed to the solvent alone (A); to 20 µM cyprodinyl (B); or to 20 µM A6 (C), and their spinal cord neurons were visualized after two days of incubation. At least nine larvae were examined for each condition, with very similar results. Arrows indicate fluorescent inclusions. Scale bars: 10 μm.

Effect of A6 on the posterior lateral line (PLL) system. (A) Scheme of a 4-dpf larva showing the posterior lateral line ganglion (PLLg) just posterior to the otic vesicle (i.e., inner ear), its central projection (CP) extending along the hindbrain, and the peripheral nerve (PLLn) extending along the horizontal myoseptum towards the sensory organs (in red), neuromasts L1–L5 and terminal neuromasts, ter (in green); (B) Lateral line ganglion in the nbt-dsred line; (C) As in (B), in a larva that had been exposed to 20 µM A6 from 1 to 3 dpf; (D) Lateral line nerve and branch to a neuromast in the nbt-dsred line; (E) Lateral line nerve in a larva that had been exposed to 20 µM A6 from 1 to 3 dpf. Arrows in (C) indicate fluorescent inclusions. Arrowheads in (D) point to individual axons within the PLL nerve; arrowheads show axonal terminations surrounding the mechanosensory hair cells of a neuromast; Scale bars: 10 µm.

Accumulation of A6 in the lateral line system of embryos that had been incubated in 50 nM A6 from 1 to 6 dpf. (A,B) Anterior trunk region; (C,D) Lateral line ganglion; (E,F) Neuromast L2; (A,C,E) Bright field; (B,D,F) Fluorescence. Arrows in (C,D) outline the PLL ganglion. Scale bars: (A) 100 μm, (C,E) 10 μm.

Acknowledgments
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