FIGURE SUMMARY
Title

Natural product derivative BIO promotes recovery after myocardial infarction via unique modulation of the cardiac microenvironment

Authors
Kim, Y.S., Jeong, H.Y., Kim, A.R., Kim, W.H., Cho, H., Um, J., Seo, Y., Kang, W.S., Jin, S.W., Kim, M.C., Kim, Y.C., Jung, D.W., Williams, D.R., Ahn, Y.
Source
Full text @ Sci. Rep.

(A) Treatment of 72 hpf Tg(cmlc2:GFP) transgenic zebrafish larvae with 2.5 µM aristolochic acid (AA) for 3 hours induces cardiomyocyte death, which can be visualized as reduced heart size in treated larvae at 168 hpf. Treatment with 5 µM BIO, but not 20 mM LiCl increased ventricular size. *P < 0.05, t-test. (B) BIO treatment completely recovered survival rate in AA-treated animals. LiCl treatment increased survival rate, but not to the same degree as BIO treated animals. *p < 0.05, t-test. (C) Micrographs of the cardiac drug-treated larvae showed that BIO increased the number of proliferating cardiomyocytes in AA-treated animals, as detected by counting BrdU-immunostained cells in the GFP-positive cardiac region (cardiomyocytes = green; BrdU positive cells = red). *p < 0.05, t-test. Scale bar = 100 µm.

Acknowledgments
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