FIGURE SUMMARY
Title

FOXN3 Regulates Hepatic Glucose Utilization

Authors
Karanth, S., Zinkhan, E.K., Hill, J.T., Yost, H.J., Schlegel, A.
Source
Full text @ Cell Rep.

FOXN3 Drives Increased Hepatic Glucose Production

(A) Human FOXN3 and zebrafish Foxn3 orthologs are shown with the central DNA-binding Forkhead box (nearly invariant aminoacyl residues 112-204) and length marked (to the right). The percent identity of the zebrafish amino-terminus, Forkhead box, and C terminus relative to human FOXN3 is shown. The conserved Sin3-binding signature (EAA within the C terminus) is marked. Putative phosphorylation sites found in five or more published phospho-proteomic surveys are shown as well: the significance of these residues’ phosphorylation has not been established in any organism.

(B) A non-transgenic (wild-type [WT]) 7 days post-fertilization (dpf) larva and two transgenic larvae were photographed in light- and green fluorescent channels in the left-lateral view. The Tg(fabp10a:foxn3,EGFP)z106 transgenic line overexpresses zebrafish foxn3 and EGFP, and the Tg(fabp10a:FOXN3,EGFP)z107 transgenic line overexpresses human FOXN3 and EGFP (transcript variant -T003). The encoded EGFP protein is translated as a free (not as a fusion) protein ( Provost et al., 2007). The scale bar represents 500 µm.

(C and D) pck1 promoter activity (bioluminescence derived from assay of luciferase) in lysates prepared from 5 and 6 days post-fertilization (dpf), never-fed Tg(pck1:Luc2,cryaa:mCherry)s952, Tg(fabp10a:foxn3,EGFP)z106; Tg(pck1:Luc2,cryaa:mCherry)s952 and Tg(fabp10a:FOXN3,EGFP)z107; and Tg(pck1:Luc2,cryaa:mCherry)s952 larvae. p < 0.02; two-tailed Student’s t test. n = 5-7.

(E and F) Whole-body glucose levels in 6 dpf WT, Tg(fabp10a: foxn3,EGFP)z106, and Tg(fabp10a:FOXN3,EGFP)z107 larval extracts. p < 0.02; two-tailed Student’s t test. n = 4-5 pooled samples of ten larvae each. All values are mean ± SEM.

(G and H) Fasting blood glucose levels in WT, Tg(fabp10a: foxn3,EGFP)z106, and Tg(fabp10a:FOXN3,EGFP)z107 transgenic adult animals of both sexes after an overnight fast.*p = 0.01; two-tailed t Student’s t test. n = 9-13.

PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 6 to Adult

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.