FIGURE SUMMARY
Title

Norepinephrine is required to promote wakefulness and for hypocretin-induced arousal in zebrafish

Authors
Singh, C., Oikonomou, G., Prober, D.A.
Source
Full text @ Elife
Fig. 1

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 2

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Day 6
Fig. 4

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Day 5 to Day 6
Fig. 4, S1

Hcrt neurons project to dbh expressing cells.

Hcrt neurons (red) project to dbh expressing cells (green) in the LC. (A) Dorsal-ventral maximum intensity projection, (B) lateral maximal intensity projection (dorsal to the right). Boxed regions are shown at higher magnification in (A′, A′′, B2). (C) Projections from Hcrt neurons to dbh-expressing neurons in the medulla oblongata (MO). A dorsal-ventral maximum intensity projection is shown. Single optical sections of the boxed regions are shown at higher magnification in (C′, C′′). Schematic diagrams indicate brain regions shown in the confocal images. a = anterior, p = posterior, d = dorsal. Scale bars = 50 µm.
Fig. 4, S4

The heat shock promoter is not suppressed in hcrtr or dbh mutants.

Representative images of brains from larvae that do not (A) or do (B) contain the hsp:Hcrt transgene, fixed 30 min after HS, and processed for ISH using a hcrt-specific probe. hcrt overexpression levels are similar for hcrtr+/- (B), hcrtr-/- (B′), dbh+/- (B′′) and dbh-/- (B′′′) animals. Larvae were generated by crossing homozygous to heterozygous mutant animals, and brains were genotyped after imaging. Dark spots in (A-A′′′) are pigment cells. Endogenous hcrt expression is not observed in (A–A′′′) because ISH development was stopped before endogenous hcrt is detectable.
Fig. 5

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 5
Fig. 6

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Fig. 8

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife
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